Long-term acclimation of anaerobic sludges for high-rate methanogenesis from LCFA

S.A. Silva, A.J. Cavaleiro, M.A. Pereira, A.J.M. Stams, M.M. Alves, D.Z. Sousa

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)


Inhibition of methanogens by long chain fatty acids (LCFA) and the low numbers of LCFA-degrading bacteria are limitations to exploit biogas production from fat-rich wastewaters. Generally reactors fail due to excessive LCFA accumulation onto the sludge. Here, long-term acclimation and bioaugmentation with a LCFA-degrading coculture were hypothesized as strategies to enhance methanogenic conversion of these compounds. Anaerobic sludges previously exposed to LCFA for more than 100 days converted a specific biomass-associated substrate of (3.2 ± 0.1) kg·kg-1 with very short lag phases (
Original languageEnglish
Pages (from-to)297-303
JournalBiomass and Bioenergy
Publication statusPublished - 2014


  • chain fatty-acids
  • oleic-acid
  • oxidizing bacteria
  • methane production
  • waste-water
  • digestion
  • lipids
  • quantification
  • hybridization
  • accumulation

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