Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies

Xiao Xiong; Yogeshwar D. Kelkar; Chris J. Geden; Chao Zhang; Yidong Wang; Evelien Jongepier; Ellen O. Martinson; Eveline C. Verhulst; Jürgen Gadau; John H. Werren; Xu Wang

doi:10.3389/fgene.2021.748135

Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies

Xiao Xiong, Yogeshwar D. Kelkar, Chris J. Geden, Chao Zhang, Yidong Wang, Evelien Jongepier, Ellen O. Martinson, Eveline C. Verhulst, Jürgen Gadau, John H. Werren, Xu Wang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

The parasitoid wasp Muscidifurax raptorellus (Hymenoptera: Pteromalidae) is a gregarious species that has received extensive attention for its potential in biological pest control against house fly, stable fly, and other filth flies. It has a high reproductive capacity and can be reared easily. However, genome assembly is not available for M. raptorellus or any other species in this genus. Previously, we assembled a complete circular mitochondrial genome with a length of 24,717 bp. Here, we assembled and annotated a high-quality nuclear genome of M. raptorellus, using a combination of long-read (104× genome coverage) and short-read (326× genome coverage) sequencing technologies. The assembled genome size is 314 Mbp in 226 contigs, with a 97.9% BUSCO completeness score and a contig N50 of 4.67 Mb, suggesting excellent continuity of this assembly. Our assembly builds the foundation for comparative and evolutionary genomic analysis in the genus of Muscidifurax and possible future biocontrol applications.

Original language	English
Article number	748135
Journal	Frontiers in Genetics
Volume	12
DOIs	https://doi.org/10.3389/fgene.2021.748135
Publication status	Published - 12 Nov 2021

Keywords

biological control
housefly
linked-read technology
Muscidifurax
PacBio sequencing
parasitoid wasp

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https://edepot.wur.nl/560009Licence: CC BY

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@article{49484223bff54b9f9621c359cd96a62b,

title = "Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies",

abstract = "The parasitoid wasp Muscidifurax raptorellus (Hymenoptera: Pteromalidae) is a gregarious species that has received extensive attention for its potential in biological pest control against house fly, stable fly, and other filth flies. It has a high reproductive capacity and can be reared easily. However, genome assembly is not available for M. raptorellus or any other species in this genus. Previously, we assembled a complete circular mitochondrial genome with a length of 24,717 bp. Here, we assembled and annotated a high-quality nuclear genome of M. raptorellus, using a combination of long-read (104× genome coverage) and short-read (326× genome coverage) sequencing technologies. The assembled genome size is 314 Mbp in 226 contigs, with a 97.9% BUSCO completeness score and a contig N50 of 4.67 Mb, suggesting excellent continuity of this assembly. Our assembly builds the foundation for comparative and evolutionary genomic analysis in the genus of Muscidifurax and possible future biocontrol applications.",

keywords = "biological control, housefly, linked-read technology, Muscidifurax, PacBio sequencing, parasitoid wasp",

author = "Xiao Xiong and Kelkar, {Yogeshwar D.} and Geden, {Chris J.} and Chao Zhang and Yidong Wang and Evelien Jongepier and Martinson, {Ellen O.} and Verhulst, {Eveline C.} and J{\"u}rgen Gadau and Werren, {John H.} and Xu Wang",

year = "2021",

month = nov,

day = "12",

doi = "10.3389/fgene.2021.748135",

language = "English",

volume = "12",

journal = "Frontiers in Genetics",

issn = "1664-8021",

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T1 - Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies

AU - Xiong, Xiao

AU - Kelkar, Yogeshwar D.

AU - Geden, Chris J.

AU - Zhang, Chao

AU - Wang, Yidong

AU - Jongepier, Evelien

AU - Martinson, Ellen O.

AU - Verhulst, Eveline C.

AU - Gadau, Jürgen

AU - Werren, John H.

AU - Wang, Xu

PY - 2021/11/12

Y1 - 2021/11/12

N2 - The parasitoid wasp Muscidifurax raptorellus (Hymenoptera: Pteromalidae) is a gregarious species that has received extensive attention for its potential in biological pest control against house fly, stable fly, and other filth flies. It has a high reproductive capacity and can be reared easily. However, genome assembly is not available for M. raptorellus or any other species in this genus. Previously, we assembled a complete circular mitochondrial genome with a length of 24,717 bp. Here, we assembled and annotated a high-quality nuclear genome of M. raptorellus, using a combination of long-read (104× genome coverage) and short-read (326× genome coverage) sequencing technologies. The assembled genome size is 314 Mbp in 226 contigs, with a 97.9% BUSCO completeness score and a contig N50 of 4.67 Mb, suggesting excellent continuity of this assembly. Our assembly builds the foundation for comparative and evolutionary genomic analysis in the genus of Muscidifurax and possible future biocontrol applications.

AB - The parasitoid wasp Muscidifurax raptorellus (Hymenoptera: Pteromalidae) is a gregarious species that has received extensive attention for its potential in biological pest control against house fly, stable fly, and other filth flies. It has a high reproductive capacity and can be reared easily. However, genome assembly is not available for M. raptorellus or any other species in this genus. Previously, we assembled a complete circular mitochondrial genome with a length of 24,717 bp. Here, we assembled and annotated a high-quality nuclear genome of M. raptorellus, using a combination of long-read (104× genome coverage) and short-read (326× genome coverage) sequencing technologies. The assembled genome size is 314 Mbp in 226 contigs, with a 97.9% BUSCO completeness score and a contig N50 of 4.67 Mb, suggesting excellent continuity of this assembly. Our assembly builds the foundation for comparative and evolutionary genomic analysis in the genus of Muscidifurax and possible future biocontrol applications.

KW - biological control

KW - housefly

KW - linked-read technology

KW - Muscidifurax

KW - PacBio sequencing

KW - parasitoid wasp

U2 - 10.3389/fgene.2021.748135

DO - 10.3389/fgene.2021.748135

M3 - Article

AN - SCOPUS:85120566309

SN - 1664-8021

VL - 12

JO - Frontiers in Genetics

JF - Frontiers in Genetics

M1 - 748135

ER -

Long-Read Assembly and Annotation of the Parasitoid Wasp Muscidifurax raptorellus, a Biological Control Agent for Filth Flies

Abstract

Keywords

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