Cowpea mosaic virus (CPMV) is a spherical, multiparticle virus. The two viral RNAs are singlestranded and replicate by a double-stranded RNA. The virus induces cytopathic structures in cells of Vigna leaves upon infection.
Structures from cells were distributed over fractions after centrifugation on discontinuous sucrose gradients. The fractions were studied by electron microscopy. The distribution of replicating RNA of CPMV among the gradient-fractions was investigated by polyacrylamide-gel electrophoresis, by molecular hybridization, by electron microscopy and by pulselabel-experiments. A correlation was shown between the amount of replicating RNA of CPMV and the amount of cytopathic structures in the gradient-fractions.
Autoradiography revealed that the RNA replication of CPMV is associated with the vesicles in this cytopathic structure.
|Qualification||Doctor of Philosophy|
|Award date||23 Jan 1974|
|Place of Publication||Wageningen|
|Publication status||Published - 1974|
- dna replication
- plant diseases
- plant viruses
- cowpea mosaic virus