Lokalisatie van de RNA-replicatie van cowpea-mozaiekvirus

A.M. Assink

Research output: Thesisinternal PhD, WU

Abstract

Cowpea mosaic virus (CPMV) is a spherical, multiparticle virus. The two viral RNAs are singlestranded and replicate by a double-stranded RNA. The virus induces cytopathic structures in cells of Vigna leaves upon infection.

Structures from cells were distributed over fractions after centrifugation on discontinuous sucrose gradients. The fractions were studied by electron microscopy. The distribution of replicating RNA of CPMV among the gradient-fractions was investigated by polyacrylamide-gel electrophoresis, by molecular hybridization, by electron microscopy and by pulselabel-experiments. A correlation was shown between the amount of replicating RNA of CPMV and the amount of cytopathic structures in the gradient-fractions.

Autoradiography revealed that the RNA replication of CPMV is associated with the vesicles in this cytopathic structure.

Original languageDutch
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • van Kammen, A., Promotor, External person
  • van der Want, J.P.H., Promotor, External person
Award date23 Jan 1974
Place of PublicationWageningen
Publisher
Print ISBNs9789022005040
Publication statusPublished - 1974

Keywords

  • replication
  • dna replication
  • plant diseases
  • plant viruses
  • vigna
  • cowpeas
  • cowpea mosaic virus

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