Localization of potato leafroll virus in leaves of secondarily-infected potato plants

J.F.J.M. van den Heuvel; C.M. de Blank; D. Peters; J.W.M. van Lent

doi:10.1007/BF01874482

Localization of potato leafroll virus in leaves of secondarily-infected potato plants

J.F.J.M. van den Heuvel, C.M. de Blank, D. Peters, J.W.M. van Lent

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Abstract

Potato leafroll virus (PLRV) antigen was localized by immunogold labelling in semi-thin leaf sections of secondarily-infected potato plants cv. Bintje. Viral antigen was present in all cell types of the phloem tissue. but occurred most abundantly in the companion cells. Detectable amounts of PLRV antigen were found only in the sieve elements in veins with a large number of infected companion cells. Occasionally, parenchyma cells were also found to be infected. PLRV was not exclusively limited to the phloem tissue in the infected potato plants, but was also found in mesophyll cells neighbouring minor phloem vessles. Spread of virus from cell to cell in the mesophyll was not observed. The distribution of PLRV in the potato leaf tissue has implication on its availability, for acquisition by aphids.

Original language	English
Pages (from-to)	567-571
Journal	European Journal of Plant Pathology
Volume	101
Issue number	5
DOIs	https://doi.org/10.1007/BF01874482
Publication status	Published - 1995

Keywords

epipolarization microscopy
immunogold labelling
luteovirus
monoclonal antibodies
silver enhancement
virus transmission

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10.1007/BF01874482

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title = "Localization of potato leafroll virus in leaves of secondarily-infected potato plants",

abstract = "Potato leafroll virus (PLRV) antigen was localized by immunogold labelling in semi-thin leaf sections of secondarily-infected potato plants cv. Bintje. Viral antigen was present in all cell types of the phloem tissue. but occurred most abundantly in the companion cells. Detectable amounts of PLRV antigen were found only in the sieve elements in veins with a large number of infected companion cells. Occasionally, parenchyma cells were also found to be infected. PLRV was not exclusively limited to the phloem tissue in the infected potato plants, but was also found in mesophyll cells neighbouring minor phloem vessles. Spread of virus from cell to cell in the mesophyll was not observed. The distribution of PLRV in the potato leaf tissue has implication on its availability, for acquisition by aphids.",

keywords = "epipolarization microscopy, immunogold labelling, luteovirus, monoclonal antibodies, silver enhancement, virus transmission",

author = "{van den Heuvel}, J.F.J.M. and {de Blank}, C.M. and D. Peters and {van Lent}, J.W.M.",

year = "1995",

doi = "10.1007/BF01874482",

language = "English",

volume = "101",

pages = "567--571",

journal = "European Journal of Plant Pathology",

issn = "0929-1873",

publisher = "Springer",

number = "5",

}

TY - JOUR

T1 - Localization of potato leafroll virus in leaves of secondarily-infected potato plants

AU - van den Heuvel, J.F.J.M.

AU - de Blank, C.M.

AU - Peters, D.

AU - van Lent, J.W.M.

PY - 1995

Y1 - 1995

N2 - Potato leafroll virus (PLRV) antigen was localized by immunogold labelling in semi-thin leaf sections of secondarily-infected potato plants cv. Bintje. Viral antigen was present in all cell types of the phloem tissue. but occurred most abundantly in the companion cells. Detectable amounts of PLRV antigen were found only in the sieve elements in veins with a large number of infected companion cells. Occasionally, parenchyma cells were also found to be infected. PLRV was not exclusively limited to the phloem tissue in the infected potato plants, but was also found in mesophyll cells neighbouring minor phloem vessles. Spread of virus from cell to cell in the mesophyll was not observed. The distribution of PLRV in the potato leaf tissue has implication on its availability, for acquisition by aphids.

AB - Potato leafroll virus (PLRV) antigen was localized by immunogold labelling in semi-thin leaf sections of secondarily-infected potato plants cv. Bintje. Viral antigen was present in all cell types of the phloem tissue. but occurred most abundantly in the companion cells. Detectable amounts of PLRV antigen were found only in the sieve elements in veins with a large number of infected companion cells. Occasionally, parenchyma cells were also found to be infected. PLRV was not exclusively limited to the phloem tissue in the infected potato plants, but was also found in mesophyll cells neighbouring minor phloem vessles. Spread of virus from cell to cell in the mesophyll was not observed. The distribution of PLRV in the potato leaf tissue has implication on its availability, for acquisition by aphids.

KW - epipolarization microscopy

KW - immunogold labelling

KW - luteovirus

KW - monoclonal antibodies

KW - silver enhancement

KW - virus transmission

U2 - 10.1007/BF01874482

DO - 10.1007/BF01874482

M3 - Article

SN - 0929-1873

VL - 101

SP - 567

EP - 571

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

IS - 5

ER -

Localization of potato leafroll virus in leaves of secondarily-infected potato plants

Abstract

Keywords

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