Abstract
Apple is generally considered as a healthy food, but 2-3% European people can not eat this fruit because it provokes allergy reaction. Four classes of apple allergen genes have been identified, they are Mal d 1, Mal d 2, Mal d 3 and Mal d 4 . This thesis focuses on the genomic characterization of these candidate genes on the apple genome by linkage mapping through sequence-specific DNA markers. In total, 26 putative Mal d genes, 25 of them were mapped on 8 linkage groups:17 for Mal d 1 , two for Mal d 2 and Mal d 3 , and four for Mal d 4 .As for the patients in northern and central Europe, the major apple allergen is Mal d 1. Genetic analysis demonstrated the Mal d 1 genes on linkage group (Chromosomes) 16 are relevant. Furthermore, a molecular marker linked to low-Mal d 1 allergenic trait was identified.
Original language | English |
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Qualification | Doctor of Philosophy |
Awarding Institution |
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Supervisors/Advisors |
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Award date | 30 Jun 2005 |
Place of Publication | Wageningen |
Print ISBNs | 9789085042426 |
DOIs | |
Publication status | Published - 30 Jun 2005 |
Keywords
- apples
- malus pumila
- allergens
- genomes
- genes
- allergies
- allergic reactions
- food allergies
- plant breeding
- varieties
- dna sequencing
- genomics