Live-imaging readouts and cell models for phenotypic profiling of mitochondrial function

Eligio F. Iannetti*, Alessandro Prigione, Jan A.M. Smeitink, Werner J.H. Koopman, Julien Beyrath, Herma Renkema

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Mitochondria are best known as the powerhouses of the cells but their cellular role goes far beyond energy production; among others, they have a pivotal function in cellular calcium and redox homeostasis. Mitochondrial dysfunction is often associated with severe and relatively rare disorders with an unmet therapeutic need. Given their central integrating role in multiple cellular pathways, mitochondrial dysfunction is also relevant in the pathogenesis of various other, more common, human pathologies. Here we discuss how live-cell high content microscopy can be used for image-based phenotypic profiling to assess mitochondrial (dys) function. From this perspective, we discuss a selection of live-cell fluorescent reporters and imaging strategies and discuss the pros/cons of human cell models in mitochondrial research. We also present an overview of live-cell high content microscopy applications used to detect disease-associated cellular phenotypes and perform cell-based drug screening.
Original languageEnglish
Article number131
JournalFrontiers in Genetics
Volume10
Issue numberMAR
DOIs
Publication statusPublished - 2019
Externally publishedYes

Keywords

  • Assay development
  • Cell models of disease
  • Cellomics
  • Fluorescent probes
  • HCS
  • Live cell microscopy
  • Mitochondrial disease
  • Pathological phenotype

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