Live imaging and quantification of cellulose biosynthesis

A.M.C. Emons, C.C. Cifuentes Espitia

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

A liquid crystal polarisation microscope (LC-Polscope) can measure birefringence of polymers quantitatively. We have set up an LC-Polscope and are able to measure single microtubules as has been shown before by Oldenbourg et al. (Biophys. J. 74: 645-654, 1998). We are using this microscope to measure the rate of synthesis of cellulose microfibrils in vitro, on living protoplasts and in the bands of xylem cells from Zinnia suspension culture. The production of cellulose microfibrils in vitro is being performed based on techniques described by Bulone et al. (J. Biol. Chem. 277,36931-36939, 2002) for which we use tobacco BY-2 suspension cells. As a standard for quantification of cellulose microfibril production, we calibrate using correlative microscopy in which the same single cellulose microfibrils, produced in vitro, or extracted from cells, are being visualised in the electron microscope prior to, or subsequently to, measurement in the LC-Polscope. Several controls are being performed, such as depolymerisation of microtubules and extraction of cell wall matrix polysaccharides
Original languageEnglish
Title of host publicationJoint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Societé Canadienne de Physiologie Végétale, 5th August - 9th August 2006, Boston, Massachusetts, USA
Pages187-187
Publication statusPublished - 2006

Fingerprint

microscopes
cellulose
image analysis
biosynthesis
microtubules
tobacco use
Zinnia
depolymerization
electron microscopes
cell suspension culture
protoplasts
xylem
microscopy
polymers
polysaccharides
cell walls
cells
synthesis
liquid crystals
methodology

Cite this

Emons, A. M. C., & Cifuentes Espitia, C. C. (2006). Live imaging and quantification of cellulose biosynthesis. In Joint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Societé Canadienne de Physiologie Végétale, 5th August - 9th August 2006, Boston, Massachusetts, USA (pp. 187-187)
Emons, A.M.C. ; Cifuentes Espitia, C.C. / Live imaging and quantification of cellulose biosynthesis. Joint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Societé Canadienne de Physiologie Végétale, 5th August - 9th August 2006, Boston, Massachusetts, USA. 2006. pp. 187-187
@inbook{b26e40b244ce46cd8d81c25ee3c302c0,
title = "Live imaging and quantification of cellulose biosynthesis",
abstract = "A liquid crystal polarisation microscope (LC-Polscope) can measure birefringence of polymers quantitatively. We have set up an LC-Polscope and are able to measure single microtubules as has been shown before by Oldenbourg et al. (Biophys. J. 74: 645-654, 1998). We are using this microscope to measure the rate of synthesis of cellulose microfibrils in vitro, on living protoplasts and in the bands of xylem cells from Zinnia suspension culture. The production of cellulose microfibrils in vitro is being performed based on techniques described by Bulone et al. (J. Biol. Chem. 277,36931-36939, 2002) for which we use tobacco BY-2 suspension cells. As a standard for quantification of cellulose microfibril production, we calibrate using correlative microscopy in which the same single cellulose microfibrils, produced in vitro, or extracted from cells, are being visualised in the electron microscope prior to, or subsequently to, measurement in the LC-Polscope. Several controls are being performed, such as depolymerisation of microtubules and extraction of cell wall matrix polysaccharides",
author = "A.M.C. Emons and {Cifuentes Espitia}, C.C.",
year = "2006",
language = "English",
pages = "187--187",
booktitle = "Joint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Societ{\'e} Canadienne de Physiologie V{\'e}g{\'e}tale, 5th August - 9th August 2006, Boston, Massachusetts, USA",

}

Emons, AMC & Cifuentes Espitia, CC 2006, Live imaging and quantification of cellulose biosynthesis. in Joint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Societé Canadienne de Physiologie Végétale, 5th August - 9th August 2006, Boston, Massachusetts, USA. pp. 187-187.

Live imaging and quantification of cellulose biosynthesis. / Emons, A.M.C.; Cifuentes Espitia, C.C.

Joint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Societé Canadienne de Physiologie Végétale, 5th August - 9th August 2006, Boston, Massachusetts, USA. 2006. p. 187-187.

Research output: Chapter in Book/Report/Conference proceedingAbstract

TY - CHAP

T1 - Live imaging and quantification of cellulose biosynthesis

AU - Emons, A.M.C.

AU - Cifuentes Espitia, C.C.

PY - 2006

Y1 - 2006

N2 - A liquid crystal polarisation microscope (LC-Polscope) can measure birefringence of polymers quantitatively. We have set up an LC-Polscope and are able to measure single microtubules as has been shown before by Oldenbourg et al. (Biophys. J. 74: 645-654, 1998). We are using this microscope to measure the rate of synthesis of cellulose microfibrils in vitro, on living protoplasts and in the bands of xylem cells from Zinnia suspension culture. The production of cellulose microfibrils in vitro is being performed based on techniques described by Bulone et al. (J. Biol. Chem. 277,36931-36939, 2002) for which we use tobacco BY-2 suspension cells. As a standard for quantification of cellulose microfibril production, we calibrate using correlative microscopy in which the same single cellulose microfibrils, produced in vitro, or extracted from cells, are being visualised in the electron microscope prior to, or subsequently to, measurement in the LC-Polscope. Several controls are being performed, such as depolymerisation of microtubules and extraction of cell wall matrix polysaccharides

AB - A liquid crystal polarisation microscope (LC-Polscope) can measure birefringence of polymers quantitatively. We have set up an LC-Polscope and are able to measure single microtubules as has been shown before by Oldenbourg et al. (Biophys. J. 74: 645-654, 1998). We are using this microscope to measure the rate of synthesis of cellulose microfibrils in vitro, on living protoplasts and in the bands of xylem cells from Zinnia suspension culture. The production of cellulose microfibrils in vitro is being performed based on techniques described by Bulone et al. (J. Biol. Chem. 277,36931-36939, 2002) for which we use tobacco BY-2 suspension cells. As a standard for quantification of cellulose microfibril production, we calibrate using correlative microscopy in which the same single cellulose microfibrils, produced in vitro, or extracted from cells, are being visualised in the electron microscope prior to, or subsequently to, measurement in the LC-Polscope. Several controls are being performed, such as depolymerisation of microtubules and extraction of cell wall matrix polysaccharides

M3 - Abstract

SP - 187

EP - 187

BT - Joint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Societé Canadienne de Physiologie Végétale, 5th August - 9th August 2006, Boston, Massachusetts, USA

ER -

Emons AMC, Cifuentes Espitia CC. Live imaging and quantification of cellulose biosynthesis. In Joint Annual Meeting of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists Societé Canadienne de Physiologie Végétale, 5th August - 9th August 2006, Boston, Massachusetts, USA. 2006. p. 187-187