Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations

K. Kots, T. Ketelaar, D.J. van den Hoogen, H.J.G. Meijer, F. Govers

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

The cytoskeleton is a dynamic but well organized intracellular network that is essential for proper functioning of eukaryotic cells. We study the cytoskeleton in Phytophthora species, oomycete plant pathogens that cause devastating diseases worldwide. We use Lifeact-eGFP expressing Phytophthora infestans for live cell imaging of the actin cytoskeleton in various developmental stages. Previously we identified actin plaques as highly immobile, long-lived structures that are unique for oomycetes. Here we present two other unique actin configurations; one associated with plug deposition in germ tubes and the
other with appressoria, infection structures formed prior to host cell penetration. Plugs are composed of cell wall material that is deposited in hyphae emerging from cysts to seal off the cytoplasm-depleted base after cytoplasm retraction towards the growing tip. Preceding plug formation a typical local actin
accumulation was observed that remained associated with the leading edge during plug deposition. In appressoria we observed an aster-like actin configuration at the contact point with the underlying surface. These findings strongly suggest a role for the actin cytoskeleton in plug formation and plant cell
penetration. For live cell imaging of the microtubule cytoskeleton we have generated a Phytophthora palmivora transformant expressing GFP-α-tubulin allowing us to visualize the dynamics of microtubules in oomycetes for the first time. The data presented here provide a better understanding of the structure
and functioning of the Phytophthora cytoskeleton. The long term goal is to uncover oomycete or Phytophthora specific features in the cytoskeleton that might be instrumental for drug design.
Original languageEnglish
Title of host publicationBook of Abstracts Oomycete Molecular Genetics Network Workshop, Pacific Grove, CA, USA 11-14 March 2017
Pages27-27
Publication statusPublished - 2017
EventOomycete Molecular Genetics Network Meeting 2017 - Asilomar Conference Grounds, Pacific Grove, CA, United States
Duration: 11 Mar 201714 Mar 2017

Conference

ConferenceOomycete Molecular Genetics Network Meeting 2017
CountryUnited States
CityPacific Grove, CA
Period11/03/1714/03/17

Fingerprint

Phytophthora
Oomycetes
Cytoskeleton
Microtubules
Actins
Actin Cytoskeleton
Cytoplasm
Phytophthora infestans
Hyphae
Drug Design
Eukaryotic Cells
Tubulin
Cell Wall
Cysts
Infection

Cite this

Kots, K., Ketelaar, T., van den Hoogen, D. J., Meijer, H. J. G., & Govers, F. (2017). Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations. In Book of Abstracts Oomycete Molecular Genetics Network Workshop, Pacific Grove, CA, USA 11-14 March 2017 (pp. 27-27)
Kots, K. ; Ketelaar, T. ; van den Hoogen, D.J. ; Meijer, H.J.G. ; Govers, F. / Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations. Book of Abstracts Oomycete Molecular Genetics Network Workshop, Pacific Grove, CA, USA 11-14 March 2017. 2017. pp. 27-27
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Kots, K, Ketelaar, T, van den Hoogen, DJ, Meijer, HJG & Govers, F 2017, Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations. in Book of Abstracts Oomycete Molecular Genetics Network Workshop, Pacific Grove, CA, USA 11-14 March 2017. pp. 27-27, Oomycete Molecular Genetics Network Meeting 2017, Pacific Grove, CA, United States, 11/03/17.

Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations. / Kots, K.; Ketelaar, T.; van den Hoogen, D.J.; Meijer, H.J.G.; Govers, F.

Book of Abstracts Oomycete Molecular Genetics Network Workshop, Pacific Grove, CA, USA 11-14 March 2017. 2017. p. 27-27.

Research output: Chapter in Book/Report/Conference proceedingAbstract

TY - CHAP

T1 - Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations

AU - Kots, K.

AU - Ketelaar, T.

AU - van den Hoogen, D.J.

AU - Meijer, H.J.G.

AU - Govers, F.

PY - 2017

Y1 - 2017

N2 - The cytoskeleton is a dynamic but well organized intracellular network that is essential for proper functioning of eukaryotic cells. We study the cytoskeleton in Phytophthora species, oomycete plant pathogens that cause devastating diseases worldwide. We use Lifeact-eGFP expressing Phytophthora infestans for live cell imaging of the actin cytoskeleton in various developmental stages. Previously we identified actin plaques as highly immobile, long-lived structures that are unique for oomycetes. Here we present two other unique actin configurations; one associated with plug deposition in germ tubes and theother with appressoria, infection structures formed prior to host cell penetration. Plugs are composed of cell wall material that is deposited in hyphae emerging from cysts to seal off the cytoplasm-depleted base after cytoplasm retraction towards the growing tip. Preceding plug formation a typical local actinaccumulation was observed that remained associated with the leading edge during plug deposition. In appressoria we observed an aster-like actin configuration at the contact point with the underlying surface. These findings strongly suggest a role for the actin cytoskeleton in plug formation and plant cellpenetration. For live cell imaging of the microtubule cytoskeleton we have generated a Phytophthora palmivora transformant expressing GFP-α-tubulin allowing us to visualize the dynamics of microtubules in oomycetes for the first time. The data presented here provide a better understanding of the structureand functioning of the Phytophthora cytoskeleton. The long term goal is to uncover oomycete or Phytophthora specific features in the cytoskeleton that might be instrumental for drug design.

AB - The cytoskeleton is a dynamic but well organized intracellular network that is essential for proper functioning of eukaryotic cells. We study the cytoskeleton in Phytophthora species, oomycete plant pathogens that cause devastating diseases worldwide. We use Lifeact-eGFP expressing Phytophthora infestans for live cell imaging of the actin cytoskeleton in various developmental stages. Previously we identified actin plaques as highly immobile, long-lived structures that are unique for oomycetes. Here we present two other unique actin configurations; one associated with plug deposition in germ tubes and theother with appressoria, infection structures formed prior to host cell penetration. Plugs are composed of cell wall material that is deposited in hyphae emerging from cysts to seal off the cytoplasm-depleted base after cytoplasm retraction towards the growing tip. Preceding plug formation a typical local actinaccumulation was observed that remained associated with the leading edge during plug deposition. In appressoria we observed an aster-like actin configuration at the contact point with the underlying surface. These findings strongly suggest a role for the actin cytoskeleton in plug formation and plant cellpenetration. For live cell imaging of the microtubule cytoskeleton we have generated a Phytophthora palmivora transformant expressing GFP-α-tubulin allowing us to visualize the dynamics of microtubules in oomycetes for the first time. The data presented here provide a better understanding of the structureand functioning of the Phytophthora cytoskeleton. The long term goal is to uncover oomycete or Phytophthora specific features in the cytoskeleton that might be instrumental for drug design.

M3 - Abstract

SP - 27

EP - 27

BT - Book of Abstracts Oomycete Molecular Genetics Network Workshop, Pacific Grove, CA, USA 11-14 March 2017

ER -

Kots K, Ketelaar T, van den Hoogen DJ, Meijer HJG, Govers F. Live cell imaging of the cytoskeleton in Phytophthora pathogens reveals unique actin and microtubule configurations. In Book of Abstracts Oomycete Molecular Genetics Network Workshop, Pacific Grove, CA, USA 11-14 March 2017. 2017. p. 27-27