Live cell imaging of meiosis in Arabidopsis thaliana

Maria A. Prusicki, Emma M. Keizer, Rik P. van Rosmalen, Shinichiro Komaki, Felix Seifert, Katja Müller, Erik Wijnker, Christian Fleck*, Arp Schnittger

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

To follow the dynamics of meiosis in the model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on the concomitant visualization of microtubules (MTs) and a meiotic cohesin subunit that allows following five cellular parameters: cell shape, MT array, nucleus position, nucleolus position, and chromatin condensation. We find that the states of these parameters are not randomly associated and identify 11 cellular states, referred to as landmarks, which occur much more frequently than closely related ones, indicating that they are convergence points during meiotic progression. As a first application of our system, we revisited a previously identified mutant in the meiotic A-type cyclin TARDY ASYNCHRONOUS MEIOSIS (TAM). Our imaging system enabled us to reveal both qualitatively and quantitatively altered landmarks in tam, foremost the formation of previously not recognized ectopic spindle- or phragmoplast-like structures that arise without attachment to chromosomes.

Original languageEnglish
Article numbere42834
JournaleLife
Volume8
DOIs
Publication statusPublished - 20 May 2019

    Fingerprint

Keywords

  • A. thaliana
  • cell biology
  • cyclin
  • development
  • meiosis
  • phragmoplast
  • plant biology
  • reproduction
  • spindle

Cite this

Prusicki, M. A., Keizer, E. M., van Rosmalen, R. P., Komaki, S., Seifert, F., Müller, K., ... Schnittger, A. (2019). Live cell imaging of meiosis in Arabidopsis thaliana. eLife, 8, [e42834]. https://doi.org/10.7554/eLife.42834