Live-Cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine

Sander Grefte, Werner J.H. Koopman*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Reactive oxygen species (ROS) play an important role in cellular (patho)physiology. Empirical evidence suggests that mitochondria are an important source of ROS, especially under pathological conditions. Here, we describe a method for ROS measurement using dihydroethidium (HEt) and live-cell microscopy.

Original languageEnglish
Title of host publicationMitochondrial Medicine
Subtitle of host publicationTargeting Mitochondria
EditorsVolkmar Weissig, Marvin Edeas
Place of PublicationNew York
PublisherHumana Press Inc.
Pages291-299
Number of pages9
Volume1
Edition2nd
ISBN (Electronic)9781071612620
ISBN (Print)9781071612613
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2275
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Fluorescence imaging
  • Mitochondrial membrane potential
  • MitoSOX Red

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