Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine

M. Forkink, P.H. Willems, W.J.H. Koopman, Sander Grefte

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

12 Citations (Scopus)


Reactive oxygen species (ROS) play an important role in both physiology and pathology. Mitochondria are an important source of the primary ROS superoxide. However, accurate detection of mitochondrial superoxide especially in living cells remains a difficult task. Here, we describe a method and the pitfalls to detect superoxide in both mitochondria and the entire cell using dihydroethidium (HEt) and live-cell microscopy.
Original languageEnglish
Title of host publicationMitochondrial Medicine
Subtitle of host publicationVolume 1 : Probing Mitochondrial Function
EditorsVolkmar Weissig, Marvin Edeas
Place of PublicationNew York
PublisherSpringer Science + Business Media
ISBN (Electronic)9781493922574
ISBN (Print)9781493922567
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer Science+Business Media


  • Live-cell assessment
  • mitochondrial reactive oxygen
  • dihydroethidine

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  • Cite this

    Forkink, M., Willems, P. H., Koopman, W. J. H., & Grefte, S. (2015). Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine. In V. Weissig, & M. Edeas (Eds.), Mitochondrial Medicine: Volume 1 : Probing Mitochondrial Function (pp. 161-169). (Methods in Molecular Biology; Vol. 1264). Springer Science + Business Media.