Liquid chromatography-electrospray ionisation-mass spectrometry based method for the determination of estradiol benzoate in hair of cattle

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Abstract

A liquid chromatography (LC)-based method with mass spectrometric (MS/MS) detection was developed for the determination of estradiol benzoate residues in hair of cattle. First hair samples were pulverized with a ball mill followed by treatment with the reducing agent Tris(2-carboxyethyl)phosphine hydrochloride (TCEP). After liquid/liquid extraction samples were further purified by solid-phase extraction. Finally samples were analyzed with LC¿MS/MS using deuterated estradiol benzoate as internal standard. The method was validated following the latest EU guidelines for screening methods using blank hair samples spiked at 5 ng g¿1. The detection capability (CCß) was less than 5 ng g¿1 and the decision limit (CC¿) was 1.6 ng g¿1. The recovery was 27% at the 5 ng g¿1 level and the accuracy was 95%. Confirmation, with two MS/MS transitions, was possible at a concentration of 5 ng g¿1 or higher. Incurred samples obtained from different animal experiments were analyzed and the presence of estradiol benzoate was confirmed. Finally hair samples from different slaughterhouses were analyzed.
Original languageEnglish
Pages (from-to)167-172
JournalAnalytica Chimica Acta
Volume529
Issue number1-2
DOIs
Publication statusPublished - 2005

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Keywords

  • gas-chromatography
  • anabolic-steroids
  • doping control
  • esters
  • testosterone
  • ethinylestradiol
  • nandrolone
  • residue
  • plasma

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