Abstract
A liquid chromatography (LC)-based method with mass spectrometric (MS/MS) detection was developed for the determination of estradiol benzoate residues in hair of cattle. First hair samples were pulverized with a ball mill followed by treatment with the reducing agent Tris(2-carboxyethyl)phosphine hydrochloride (TCEP). After liquid/liquid extraction samples were further purified by solid-phase extraction. Finally samples were analyzed with LC¿MS/MS using deuterated estradiol benzoate as internal standard. The method was validated following the latest EU guidelines for screening methods using blank hair samples spiked at 5 ng g¿1. The detection capability (CCß) was less than 5 ng g¿1 and the decision limit (CC¿) was 1.6 ng g¿1. The recovery was 27% at the 5 ng g¿1 level and the accuracy was 95%. Confirmation, with two MS/MS transitions, was possible at a concentration of 5 ng g¿1 or higher. Incurred samples obtained from different animal experiments were analyzed and the presence of estradiol benzoate was confirmed. Finally hair samples from different slaughterhouses were analyzed.
| Original language | English |
|---|---|
| Pages (from-to) | 167-172 |
| Journal | Analytica Chimica Acta |
| Volume | 529 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2005 |
Keywords
- gas-chromatography
- anabolic-steroids
- doping control
- esters
- testosterone
- ethinylestradiol
- nandrolone
- residue
- plasma