Liquid chromatographic method to determine narasin in feedingstuffs and premixtures: development, validation and interlaboratory study

A. Thalman, K. Wagner, M.J.H. Tomassen, J.J.M. Driessen, J. de Jong

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9 Citations (Scopus)

Abstract

A reversed-phase liquid chromatography (LC) method for narasin in feedingstuffs and premixtures was developed, validated, and interlaboratory studied. The extraction solvent was methanol-K2HPO4 solution (9 + 1, v/v). Narasin was detected at 600 nm after post-column derivatization with dimethylamino-benzaldehyde. Recovery was >90%. The repeatability (RSDr) in feed (20-140 mg/kg) ranged between 1.2 and 10.5%; the within-laboratory reproducibility (RSD(R)) ranged between 2.2 and 4.9%. The limit of determination was
Original languageEnglish
Pages (from-to)1278-1286
JournalJournal of AOAC International
Volume87
Issue number6
Publication statusPublished - 2004

Keywords

  • derivatization

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