A reversed-phase liquid chromatography (LC) method for narasin in feedingstuffs and premixtures was developed, validated, and interlaboratory studied. The extraction solvent was methanol-K2HPO4 solution (9 + 1, v/v). Narasin was detected at 600 nm after post-column derivatization with dimethylamino-benzaldehyde. Recovery was >90%. The repeatability (RSDr) in feed (20-140 mg/kg) ranged between 1.2 and 10.5%; the within-laboratory reproducibility (RSD(R)) ranged between 2.2 and 4.9%. The limit of determination was
|Journal||Journal of AOAC International|
|Publication status||Published - 2004|
Thalman, A., Wagner, K., Tomassen, M. J. H., Driessen, J. J. M., & de Jong, J. (2004). Liquid chromatographic method to determine narasin in feedingstuffs and premixtures: development, validation and interlaboratory study. Journal of AOAC International, 87(6), 1278-1286.