Liquid Chromatographic Determination of Alternaria Toxins in Carrots

M. Solfrizzo, A. De Girolamo, C. Vitti, R.W. van den Bulk

    Research output: Contribution to journalArticleAcademicpeer-review

    47 Citations (Scopus)

    Abstract

    A liquid chromatographic (LC) method was developed for the determination of Alternaria radicina and A. alternata toxins in carrots. Toxins were extracted from carrot with an acidified mixture of water¿methanol¿acetonitrile. The filtered extract was divided in 2 parts that were purified by solid-phase extraction on a C18 column for the analysis of radicinin (RAD), altertoxin-I (ATX-I), alternariol (AOH), and alternariol methyl ether (AME), and on a polymeric Oasis® HLB column for tenuazonic acid (TeA). Toxins were quantified by reversed-phase LC with UV diode array detection by using 2 consecutive isocratic mixtures of acetonitrile¿sodium dihydrogen phosphate solution. Mean recoveries of TeA, ATX-I, AME, RAD, and AOH from carrots spiked at levels between 0.5 and 3.0 ¿g/g were 69, 71, 90, 36, and 78%, with mean within-laboratory repeatability of 14, 5, 4, 6, and 18%, respectively. The mean between-laboratory reproducibilities for the determination of TeA, ATX-I, AME, and RAD in spiked samples were 25, 22, 6, and 12%, respectively. Limits of detection (signal-to-noise ratio of 3) for RAD, TeA, ATX-I, AME, and AOH were 0.006, 0.02, 0.02, 0.01, and 0.005 ¿g/g, respectively. RAD was detected (0.16¿13.9 ¿g/g) in 3 out of 266 carrot samples produced under organic conditions in 3 European locations, whereas A. alternata mycotoxins were not found in any tested samples
    Original languageEnglish
    Pages (from-to)101-106
    JournalJournal of AOAC International
    Volume87
    Issue number1
    Publication statusPublished - 2004

    Fingerprint

    tenuazonic acid
    Tenuazonic Acid
    Alternaria
    Daucus carota
    Methyl Ethers
    ether
    carrots
    toxin
    ethers
    toxins
    liquid
    liquids
    acid
    Liquids
    Alternaria radicina
    oases
    Alternaria alternata
    oasis
    solid phase extraction
    sampling

    Keywords

    • mycotoxins
    • radicina
    • pcr

    Cite this

    Solfrizzo, M. ; De Girolamo, A. ; Vitti, C. ; van den Bulk, R.W. / Liquid Chromatographic Determination of Alternaria Toxins in Carrots. In: Journal of AOAC International. 2004 ; Vol. 87, No. 1. pp. 101-106.
    @article{abe754fe24b848d58cf41f3db71958be,
    title = "Liquid Chromatographic Determination of Alternaria Toxins in Carrots",
    abstract = "A liquid chromatographic (LC) method was developed for the determination of Alternaria radicina and A. alternata toxins in carrots. Toxins were extracted from carrot with an acidified mixture of water¿methanol¿acetonitrile. The filtered extract was divided in 2 parts that were purified by solid-phase extraction on a C18 column for the analysis of radicinin (RAD), altertoxin-I (ATX-I), alternariol (AOH), and alternariol methyl ether (AME), and on a polymeric Oasis{\circledR} HLB column for tenuazonic acid (TeA). Toxins were quantified by reversed-phase LC with UV diode array detection by using 2 consecutive isocratic mixtures of acetonitrile¿sodium dihydrogen phosphate solution. Mean recoveries of TeA, ATX-I, AME, RAD, and AOH from carrots spiked at levels between 0.5 and 3.0 ¿g/g were 69, 71, 90, 36, and 78{\%}, with mean within-laboratory repeatability of 14, 5, 4, 6, and 18{\%}, respectively. The mean between-laboratory reproducibilities for the determination of TeA, ATX-I, AME, and RAD in spiked samples were 25, 22, 6, and 12{\%}, respectively. Limits of detection (signal-to-noise ratio of 3) for RAD, TeA, ATX-I, AME, and AOH were 0.006, 0.02, 0.02, 0.01, and 0.005 ¿g/g, respectively. RAD was detected (0.16¿13.9 ¿g/g) in 3 out of 266 carrot samples produced under organic conditions in 3 European locations, whereas A. alternata mycotoxins were not found in any tested samples",
    keywords = "mycotoxins, radicina, pcr",
    author = "M. Solfrizzo and {De Girolamo}, A. and C. Vitti and {van den Bulk}, R.W.",
    year = "2004",
    language = "English",
    volume = "87",
    pages = "101--106",
    journal = "Journal of AOAC International",
    issn = "1060-3271",
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    Solfrizzo, M, De Girolamo, A, Vitti, C & van den Bulk, RW 2004, 'Liquid Chromatographic Determination of Alternaria Toxins in Carrots' Journal of AOAC International, vol. 87, no. 1, pp. 101-106.

    Liquid Chromatographic Determination of Alternaria Toxins in Carrots. / Solfrizzo, M.; De Girolamo, A.; Vitti, C.; van den Bulk, R.W.

    In: Journal of AOAC International, Vol. 87, No. 1, 2004, p. 101-106.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Liquid Chromatographic Determination of Alternaria Toxins in Carrots

    AU - Solfrizzo, M.

    AU - De Girolamo, A.

    AU - Vitti, C.

    AU - van den Bulk, R.W.

    PY - 2004

    Y1 - 2004

    N2 - A liquid chromatographic (LC) method was developed for the determination of Alternaria radicina and A. alternata toxins in carrots. Toxins were extracted from carrot with an acidified mixture of water¿methanol¿acetonitrile. The filtered extract was divided in 2 parts that were purified by solid-phase extraction on a C18 column for the analysis of radicinin (RAD), altertoxin-I (ATX-I), alternariol (AOH), and alternariol methyl ether (AME), and on a polymeric Oasis® HLB column for tenuazonic acid (TeA). Toxins were quantified by reversed-phase LC with UV diode array detection by using 2 consecutive isocratic mixtures of acetonitrile¿sodium dihydrogen phosphate solution. Mean recoveries of TeA, ATX-I, AME, RAD, and AOH from carrots spiked at levels between 0.5 and 3.0 ¿g/g were 69, 71, 90, 36, and 78%, with mean within-laboratory repeatability of 14, 5, 4, 6, and 18%, respectively. The mean between-laboratory reproducibilities for the determination of TeA, ATX-I, AME, and RAD in spiked samples were 25, 22, 6, and 12%, respectively. Limits of detection (signal-to-noise ratio of 3) for RAD, TeA, ATX-I, AME, and AOH were 0.006, 0.02, 0.02, 0.01, and 0.005 ¿g/g, respectively. RAD was detected (0.16¿13.9 ¿g/g) in 3 out of 266 carrot samples produced under organic conditions in 3 European locations, whereas A. alternata mycotoxins were not found in any tested samples

    AB - A liquid chromatographic (LC) method was developed for the determination of Alternaria radicina and A. alternata toxins in carrots. Toxins were extracted from carrot with an acidified mixture of water¿methanol¿acetonitrile. The filtered extract was divided in 2 parts that were purified by solid-phase extraction on a C18 column for the analysis of radicinin (RAD), altertoxin-I (ATX-I), alternariol (AOH), and alternariol methyl ether (AME), and on a polymeric Oasis® HLB column for tenuazonic acid (TeA). Toxins were quantified by reversed-phase LC with UV diode array detection by using 2 consecutive isocratic mixtures of acetonitrile¿sodium dihydrogen phosphate solution. Mean recoveries of TeA, ATX-I, AME, RAD, and AOH from carrots spiked at levels between 0.5 and 3.0 ¿g/g were 69, 71, 90, 36, and 78%, with mean within-laboratory repeatability of 14, 5, 4, 6, and 18%, respectively. The mean between-laboratory reproducibilities for the determination of TeA, ATX-I, AME, and RAD in spiked samples were 25, 22, 6, and 12%, respectively. Limits of detection (signal-to-noise ratio of 3) for RAD, TeA, ATX-I, AME, and AOH were 0.006, 0.02, 0.02, 0.01, and 0.005 ¿g/g, respectively. RAD was detected (0.16¿13.9 ¿g/g) in 3 out of 266 carrot samples produced under organic conditions in 3 European locations, whereas A. alternata mycotoxins were not found in any tested samples

    KW - mycotoxins

    KW - radicina

    KW - pcr

    M3 - Article

    VL - 87

    SP - 101

    EP - 106

    JO - Journal of AOAC International

    JF - Journal of AOAC International

    SN - 1060-3271

    IS - 1

    ER -