Lipochito-oligosaccharides re-initiate root hair tip growth in Vicia sativa with high calcium and spectrin-like antigen at the tip

N.C.A. de Ruijter, M.B. Rook, T. Bisseling, A.M.C. Emons

Research output: Contribution to journalArticleAcademicpeer-review

105 Citations (Scopus)

Abstract

Lipochitooligosaccharides, Nod factors secreted by Rhizobium bacteria, are signal molecules that induce deformation of root hairs of their host plant. A bioassay was used for deformation, and the cytological changes induced by specific lipochitooligosaccharides in root hairs of Vicia sativa L. (vetch), grown between glass slides, were examined. In the assay, root hairs of a particular developmental stage, those that were terminating growth, were susceptible to deformation. These hairs obtained characteristics of tipgrowing cells again: (i) a polar cytoplasmic organization and reverse fountain streaming, (ii) an accumulation of a spectrinlike antigen at the tip, and (iii) a tipfocused calcium gradient. Calcium gradients were visualized in Indo1 loaded root hairs with UV confocal microscopy and ratioimaging. The results show that hairs respond to the bacterial signal by recovering cytoplasmic polarity and exocytosis
Original languageEnglish
Pages (from-to)341-350
JournalThe Plant Journal
Volume13
Issue number3
DOIs
Publication statusPublished - 1998

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