Lipid recovery from deep eutectic solvents by polar antisolvents

Calvin Lo, René H. Wijffels, Michel H.M. Eppink*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)


Deep eutectic solvents (DES) can be a green alternative extraction technology for microalgae lipids, acting simultaneously as a pre-treatment agent and solvent. However, due to the low vapor pressure of both DES and lipid solute, the recovery of lipids and solvent regeneration remains difficult. In this study, we developed a novel strategy to separate the dissolved sunflower and model algae oil from imidazole/hexanoic acid DES by using polar antisolvents (water, methanol, and ethanol). The polarity and the amount of antisolvent influenced the lipid solubility in DES. While the water was the strongest antisolvent, the alcohols were easier to evaporate, ensuring easy DES regeneration. By adding small amounts of water and methanol, more than 90% of the lipids were recovered in the form of high-purity oils (>90%). In the case of ethanol, a large amount of ethanol was required, which diluted the solvent-rich phase and solubilized more lipids in it. Based on three repeated cycles with the selected antisolvent methanol, > 90% of the eutectic solvent could be regenerated.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalFood and Bioproducts Processing
Early online dateJan 2024
Publication statusPublished - Jan 2024


  • Deep eutectic solvent (DES) regeneration
  • Deep eutectic solvents (DES)
  • Lipid recovery
  • Polar antisolvents


Dive into the research topics of 'Lipid recovery from deep eutectic solvents by polar antisolvents'. Together they form a unique fingerprint.

Cite this