Lipid affinity beads: From identifying new lipid binding proteins to assessing their binding properties

Fionn McLoughlin, Christa Testerink

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets.

Original languageEnglish
Title of host publicationPlant Lipid Signaling Protocols
EditorsTeun Munnik, Ingo Heilmann
Place of PublicationTotowa
PublisherSpringer – Humana Press
Pages273-280
Number of pages8
ISBN (Electronic)9781627034012
ISBN (Print)9781627034005
DOIs
Publication statusPublished - 4 Sep 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherSpringer - Humana Press
Volume1009
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Lipid Â-binding
  • Phosphatidic acid
  • Phosphatidic acid beads
  • Phosphatidic acid target proteins
  • Phospholipid signaling

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