Linoleic acid stimulation results in TGF-β1 production and inhibition of PEDV infection in vitro

Shanshan Yang; Caiying Wang; Xin Huang; Christine A. Jansen; Huub F.J. Savelkoul; Guangliang Liu

doi:10.1016/j.virol.2023.03.004

Linoleic acid stimulation results in TGF-β1 production and inhibition of PEDV infection in vitro

Shanshan Yang
, Caiying Wang
, Xin Huang
, Christine A. Jansen
, Huub F.J. Savelkoul
, Guangliang Liu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

Linoleic acid (LA) is recommended to improve pork quality. However, whether it affects the intestinal immune response in pigs is still unclear. Our ex vivo experiments demonstrated that LA stimulation resulted in increased frequencies of Tregs in PBMCs but not in Peyer's Patches (PPs). The results of RT-qPCR, flow cytometry, and ELISA indicated that LA increased the TGF-β1 expression level in DCs isolated from PEDV infected pigs. Furthermore, RT-qPCR and flow cytometry results demonstrated that TGF-β1 was associated with higher frequencies of Tregs both in PBMCs and PPs. Additional investigations showed that TGF-β1 inhibited PEDV infection in vitro. Besides, knocking-out TGF-β1 in IPEC-J2 cells resulted in higher viral load. Taken together, our results demonstrated that LA stimulation resulted in enhanced production of TGF-β1 by DC, which resulted in higher frequencies of Tregs production and inhibition of PEDV infection.

Original language	English
Pages (from-to)	89-96
Journal	Virology
Volume	581
DOIs	https://doi.org/10.1016/j.virol.2023.03.004
Publication status	Published - Apr 2023

Keywords

Anti-inflammatory response
Linoleic acid
PEDV
TGF-β1
Tregs

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@article{8db19200685a4916a1a7432dad51ce36,

title = "Linoleic acid stimulation results in TGF-β1 production and inhibition of PEDV infection in vitro",

abstract = "Linoleic acid (LA) is recommended to improve pork quality. However, whether it affects the intestinal immune response in pigs is still unclear. Our ex vivo experiments demonstrated that LA stimulation resulted in increased frequencies of Tregs in PBMCs but not in Peyer's Patches (PPs). The results of RT-qPCR, flow cytometry, and ELISA indicated that LA increased the TGF-β1 expression level in DCs isolated from PEDV infected pigs. Furthermore, RT-qPCR and flow cytometry results demonstrated that TGF-β1 was associated with higher frequencies of Tregs both in PBMCs and PPs. Additional investigations showed that TGF-β1 inhibited PEDV infection in vitro. Besides, knocking-out TGF-β1 in IPEC-J2 cells resulted in higher viral load. Taken together, our results demonstrated that LA stimulation resulted in enhanced production of TGF-β1 by DC, which resulted in higher frequencies of Tregs production and inhibition of PEDV infection.",

keywords = "Anti-inflammatory response, Linoleic acid, PEDV, TGF-β1, Tregs",

author = "Shanshan Yang and Caiying Wang and Xin Huang and Jansen, \{Christine A.\} and Savelkoul, \{Huub F.J.\} and Guangliang Liu",

year = "2023",

month = apr,

doi = "10.1016/j.virol.2023.03.004",

language = "English",

volume = "581",

pages = "89--96",

journal = "Virology",

issn = "0042-6822",

publisher = "Elsevier",

}

TY - JOUR

T1 - Linoleic acid stimulation results in TGF-β1 production and inhibition of PEDV infection in vitro

AU - Yang, Shanshan

AU - Wang, Caiying

AU - Huang, Xin

AU - Jansen, Christine A.

AU - Savelkoul, Huub F.J.

AU - Liu, Guangliang

PY - 2023/4

Y1 - 2023/4

N2 - Linoleic acid (LA) is recommended to improve pork quality. However, whether it affects the intestinal immune response in pigs is still unclear. Our ex vivo experiments demonstrated that LA stimulation resulted in increased frequencies of Tregs in PBMCs but not in Peyer's Patches (PPs). The results of RT-qPCR, flow cytometry, and ELISA indicated that LA increased the TGF-β1 expression level in DCs isolated from PEDV infected pigs. Furthermore, RT-qPCR and flow cytometry results demonstrated that TGF-β1 was associated with higher frequencies of Tregs both in PBMCs and PPs. Additional investigations showed that TGF-β1 inhibited PEDV infection in vitro. Besides, knocking-out TGF-β1 in IPEC-J2 cells resulted in higher viral load. Taken together, our results demonstrated that LA stimulation resulted in enhanced production of TGF-β1 by DC, which resulted in higher frequencies of Tregs production and inhibition of PEDV infection.

AB - Linoleic acid (LA) is recommended to improve pork quality. However, whether it affects the intestinal immune response in pigs is still unclear. Our ex vivo experiments demonstrated that LA stimulation resulted in increased frequencies of Tregs in PBMCs but not in Peyer's Patches (PPs). The results of RT-qPCR, flow cytometry, and ELISA indicated that LA increased the TGF-β1 expression level in DCs isolated from PEDV infected pigs. Furthermore, RT-qPCR and flow cytometry results demonstrated that TGF-β1 was associated with higher frequencies of Tregs both in PBMCs and PPs. Additional investigations showed that TGF-β1 inhibited PEDV infection in vitro. Besides, knocking-out TGF-β1 in IPEC-J2 cells resulted in higher viral load. Taken together, our results demonstrated that LA stimulation resulted in enhanced production of TGF-β1 by DC, which resulted in higher frequencies of Tregs production and inhibition of PEDV infection.

KW - Anti-inflammatory response

KW - Linoleic acid

KW - PEDV

KW - TGF-β1

KW - Tregs

U2 - 10.1016/j.virol.2023.03.004

DO - 10.1016/j.virol.2023.03.004

M3 - Article

AN - SCOPUS:85150019994

SN - 0042-6822

VL - 581

SP - 89

EP - 96

JO - Virology

JF - Virology

ER -

Linoleic acid stimulation results in TGF-β1 production and inhibition of PEDV infection in vitro

Abstract

Keywords

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