Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples.

W.H.M. van der Poel; B. Cay; S. Zientara; F. Steinbach; J.F. Valarcher; A. Botner; M.H. Mars; R.W. van der Hakze-van der Honing; H. Schirrmeier; M. Beer

doi:10.1136/vr.102180

Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples.

W.H.M. van der Poel, B. Cay, S. Zientara, F. Steinbach, J.F. Valarcher, A. Botner, M.H. Mars, R.W. van der Hakze-van der Honing, H. Schirrmeier, M. Beer

Virology & Molecular Biology

Research output: Contribution to journal › Article › Academic › peer-review

23 Citations (Scopus)

Abstract

Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories. All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.

Original language	English
Article number	380
Number of pages	3
Journal	Veterinary Record
Volume	174
DOIs	https://doi.org/10.1136/vr.102180
Publication status	Published - 2014

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title = "Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples.",

abstract = "Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories. All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.",

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doi = "10.1136/vr.102180",

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T1 - Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples.

AU - van der Poel, W.H.M.

AU - Cay, B.

AU - Zientara, S.

AU - Steinbach, F.

AU - Valarcher, J.F.

AU - Botner, A.

AU - Mars, M.H.

AU - van der Hakze-van der Honing, R.W.

AU - Schirrmeier, H.

AU - Beer, M.

PY - 2014

Y1 - 2014

N2 - Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories. All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.

AB - Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories. All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.

U2 - 10.1136/vr.102180

DO - 10.1136/vr.102180

M3 - Article

SN - 0042-4900

VL - 174

JO - Veterinary Record

JF - Veterinary Record

M1 - 380

ER -

Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples.

Abstract

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