Limited changes in the fecal microbiome composition of laying hens after oral inoculation with wild duck feces

Janneke Schreuder, Francisca C. Velkers, Ruth J. Bouwstra, N. Beerens, J.A. Stegeman, Willem F. de Boer, A.R.W. Elbers, Pim van Hooft, Anneke Feberwee, A. Bossers, Stephanie D. Jurburg

Research output: Contribution to journalArticleProfessional

1 Citation (Scopus)

Abstract

Interspecies transmission of fecal microbiota can serve as an indicator for (indirect) contact between domestic and wild animals to assess risks of pathogen transmission, e.g., avian influenza. Here, we investigated whether oral inoculation of laying hens with feces of wild ducks (mallards, Anas platyrhynchos) resulted in a hen fecal microbiome that was detectably altered on community parameters or relative abundances of individual genera. To distinguish between effects of the duck inoculum and effects of the inoculation procedure, we compared the fecal microbiomes of adult laying hens resulting from 3 treatments: inoculation with wild duck feces (duck), inoculation with chicken feces (auto), and a negative control group with no treatment. We collected cloacal swabs from 7 hens per treatment before (day 0), and 2 and 7 D after inoculation, and performed 16S rRNA amplicon sequencing. No distinguishable effect of inoculation with duck feces on microbiome community (alpha and beta diversity) was found compared to auto or control treatments. At the individual taxonomic level, the relative abundance of the genus Alistipes (phylum Bacteroidetes) was significantly higher in the inoculated treatments (auto and duck) compared to the control 2 D after inoculation. Seven days after inoculation, the relative abundance of Alistipes had increased in the control and no effect was found anymore across treatments. These effects might be explained by the perturbation of the hen's microbiome caused by the inoculation procedure itself, or by intrinsic temporal variation in the hen's microbiome. This experiment shows that a single inoculation of fecal microbiota from duck feces to laying hens did not cause a measurable alteration of the gut microbiome community. Furthermore, the temporary change in relative abundance for Alistipes could not be attributed to the duck feces inoculation. These outcomes suggest that the fecal microbiome of adult laying hens may not be a useful indicator for detection of single oral exposure to wild duck feces.

Original languageEnglish
Pages (from-to)6542–6551
JournalPoultry Science
Volume98
Issue number12
DOIs
Publication statusPublished - 2019

Fingerprint

laying hens
ducks
oral administration
feces
hens
automobiles
Anas platyrhynchos
indirect contact
microbiome
avian influenza
wild animals
domestic animals
temporal variation
inoculum
digestive system
ribosomal RNA
chickens
pathogens

Cite this

Schreuder, J., Velkers, F. C., Bouwstra, R. J., Beerens, N., Stegeman, J. A., de Boer, W. F., ... Jurburg, S. D. (2019). Limited changes in the fecal microbiome composition of laying hens after oral inoculation with wild duck feces. Poultry Science, 98(12), 6542–6551. https://doi.org/10.3382/ps/pez526
Schreuder, Janneke ; Velkers, Francisca C. ; Bouwstra, Ruth J. ; Beerens, N. ; Stegeman, J.A. ; de Boer, Willem F. ; Elbers, A.R.W. ; van Hooft, Pim ; Feberwee, Anneke ; Bossers, A. ; Jurburg, Stephanie D. . / Limited changes in the fecal microbiome composition of laying hens after oral inoculation with wild duck feces. In: Poultry Science. 2019 ; Vol. 98, No. 12. pp. 6542–6551.
@article{92c53a35852541238bbcc492ce50fa9b,
title = "Limited changes in the fecal microbiome composition of laying hens after oral inoculation with wild duck feces",
abstract = "Interspecies transmission of fecal microbiota can serve as an indicator for (indirect) contact between domestic and wild animals to assess risks of pathogen transmission, e.g., avian influenza. Here, we investigated whether oral inoculation of laying hens with feces of wild ducks (mallards, Anas platyrhynchos) resulted in a hen fecal microbiome that was detectably altered on community parameters or relative abundances of individual genera. To distinguish between effects of the duck inoculum and effects of the inoculation procedure, we compared the fecal microbiomes of adult laying hens resulting from 3 treatments: inoculation with wild duck feces (duck), inoculation with chicken feces (auto), and a negative control group with no treatment. We collected cloacal swabs from 7 hens per treatment before (day 0), and 2 and 7 D after inoculation, and performed 16S rRNA amplicon sequencing. No distinguishable effect of inoculation with duck feces on microbiome community (alpha and beta diversity) was found compared to auto or control treatments. At the individual taxonomic level, the relative abundance of the genus Alistipes (phylum Bacteroidetes) was significantly higher in the inoculated treatments (auto and duck) compared to the control 2 D after inoculation. Seven days after inoculation, the relative abundance of Alistipes had increased in the control and no effect was found anymore across treatments. These effects might be explained by the perturbation of the hen's microbiome caused by the inoculation procedure itself, or by intrinsic temporal variation in the hen's microbiome. This experiment shows that a single inoculation of fecal microbiota from duck feces to laying hens did not cause a measurable alteration of the gut microbiome community. Furthermore, the temporary change in relative abundance for Alistipes could not be attributed to the duck feces inoculation. These outcomes suggest that the fecal microbiome of adult laying hens may not be a useful indicator for detection of single oral exposure to wild duck feces.",
author = "Janneke Schreuder and Velkers, {Francisca C.} and Bouwstra, {Ruth J.} and N. Beerens and J.A. Stegeman and {de Boer}, {Willem F.} and A.R.W. Elbers and {van Hooft}, Pim and Anneke Feberwee and A. Bossers and Jurburg, {Stephanie D.}",
year = "2019",
doi = "10.3382/ps/pez526",
language = "English",
volume = "98",
pages = "6542–6551",
journal = "Poultry Science",
issn = "0032-5791",
publisher = "Oxford University Press",
number = "12",

}

Schreuder, J, Velkers, FC, Bouwstra, RJ, Beerens, N, Stegeman, JA, de Boer, WF, Elbers, ARW, van Hooft, P, Feberwee, A, Bossers, A & Jurburg, SD 2019, 'Limited changes in the fecal microbiome composition of laying hens after oral inoculation with wild duck feces', Poultry Science, vol. 98, no. 12, pp. 6542–6551. https://doi.org/10.3382/ps/pez526

Limited changes in the fecal microbiome composition of laying hens after oral inoculation with wild duck feces. / Schreuder, Janneke; Velkers, Francisca C.; Bouwstra, Ruth J.; Beerens, N.; Stegeman, J.A.; de Boer, Willem F.; Elbers, A.R.W.; van Hooft, Pim; Feberwee, Anneke ; Bossers, A.; Jurburg, Stephanie D. .

In: Poultry Science, Vol. 98, No. 12, 2019, p. 6542–6551.

Research output: Contribution to journalArticleProfessional

TY - JOUR

T1 - Limited changes in the fecal microbiome composition of laying hens after oral inoculation with wild duck feces

AU - Schreuder, Janneke

AU - Velkers, Francisca C.

AU - Bouwstra, Ruth J.

AU - Beerens, N.

AU - Stegeman, J.A.

AU - de Boer, Willem F.

AU - Elbers, A.R.W.

AU - van Hooft, Pim

AU - Feberwee, Anneke

AU - Bossers, A.

AU - Jurburg, Stephanie D.

PY - 2019

Y1 - 2019

N2 - Interspecies transmission of fecal microbiota can serve as an indicator for (indirect) contact between domestic and wild animals to assess risks of pathogen transmission, e.g., avian influenza. Here, we investigated whether oral inoculation of laying hens with feces of wild ducks (mallards, Anas platyrhynchos) resulted in a hen fecal microbiome that was detectably altered on community parameters or relative abundances of individual genera. To distinguish between effects of the duck inoculum and effects of the inoculation procedure, we compared the fecal microbiomes of adult laying hens resulting from 3 treatments: inoculation with wild duck feces (duck), inoculation with chicken feces (auto), and a negative control group with no treatment. We collected cloacal swabs from 7 hens per treatment before (day 0), and 2 and 7 D after inoculation, and performed 16S rRNA amplicon sequencing. No distinguishable effect of inoculation with duck feces on microbiome community (alpha and beta diversity) was found compared to auto or control treatments. At the individual taxonomic level, the relative abundance of the genus Alistipes (phylum Bacteroidetes) was significantly higher in the inoculated treatments (auto and duck) compared to the control 2 D after inoculation. Seven days after inoculation, the relative abundance of Alistipes had increased in the control and no effect was found anymore across treatments. These effects might be explained by the perturbation of the hen's microbiome caused by the inoculation procedure itself, or by intrinsic temporal variation in the hen's microbiome. This experiment shows that a single inoculation of fecal microbiota from duck feces to laying hens did not cause a measurable alteration of the gut microbiome community. Furthermore, the temporary change in relative abundance for Alistipes could not be attributed to the duck feces inoculation. These outcomes suggest that the fecal microbiome of adult laying hens may not be a useful indicator for detection of single oral exposure to wild duck feces.

AB - Interspecies transmission of fecal microbiota can serve as an indicator for (indirect) contact between domestic and wild animals to assess risks of pathogen transmission, e.g., avian influenza. Here, we investigated whether oral inoculation of laying hens with feces of wild ducks (mallards, Anas platyrhynchos) resulted in a hen fecal microbiome that was detectably altered on community parameters or relative abundances of individual genera. To distinguish between effects of the duck inoculum and effects of the inoculation procedure, we compared the fecal microbiomes of adult laying hens resulting from 3 treatments: inoculation with wild duck feces (duck), inoculation with chicken feces (auto), and a negative control group with no treatment. We collected cloacal swabs from 7 hens per treatment before (day 0), and 2 and 7 D after inoculation, and performed 16S rRNA amplicon sequencing. No distinguishable effect of inoculation with duck feces on microbiome community (alpha and beta diversity) was found compared to auto or control treatments. At the individual taxonomic level, the relative abundance of the genus Alistipes (phylum Bacteroidetes) was significantly higher in the inoculated treatments (auto and duck) compared to the control 2 D after inoculation. Seven days after inoculation, the relative abundance of Alistipes had increased in the control and no effect was found anymore across treatments. These effects might be explained by the perturbation of the hen's microbiome caused by the inoculation procedure itself, or by intrinsic temporal variation in the hen's microbiome. This experiment shows that a single inoculation of fecal microbiota from duck feces to laying hens did not cause a measurable alteration of the gut microbiome community. Furthermore, the temporary change in relative abundance for Alistipes could not be attributed to the duck feces inoculation. These outcomes suggest that the fecal microbiome of adult laying hens may not be a useful indicator for detection of single oral exposure to wild duck feces.

U2 - 10.3382/ps/pez526

DO - 10.3382/ps/pez526

M3 - Article

VL - 98

SP - 6542

EP - 6551

JO - Poultry Science

JF - Poultry Science

SN - 0032-5791

IS - 12

ER -