Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability

N. Ntagkas, E.J. Woltering, Sofoklis Bouras, C.H. de Vos, J.A. Dieleman, Celine Nicole, C.W. Labrie, L.F.M. Marcelis

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

L-ascorbate (ASC) is essential for human health. Therefore, there is interest in increasing the ASC content of crops like tomato. High irradiance induces accumulation of ASC in green tomato fruits. The D-mannose/L-galactose biosynthetic pathway accounts for the most ASC in plants. The myo-inositol and galacturonate pathways have been proposed to exist but never identified in plants. The D-mannose/L-galactose starts from D-glucose. In a series of experiments, we tested the hypothesis that ASC levels depend on soluble carbohydrate content when tomato fruits ripen under irradiances that stimulate ASC biosynthesis. We show that ASC levels considerably increased when fruits ripened under light, but carbohydrate levels did not show a parallel increase. When carbohydrate levels in fruits were altered by flower pruning, no effects on
ASC levels were observed at harvest or after ripening under irradiances that induce ASC accumulation. Artificial feeding of trusses with sucrose increased carbohydrate levels, but did not affect the light-induced ASC levels. We conclude that light-induced accumulation of ASC is independent of the carbohydrate content in tomato fruits. In tomato fruit treated with light, the increase in ASC was preceded by a concomitant increase in myo-inositol.
LanguageEnglish
Article number86
JournalPlants
Volume8
Issue number4
DOIs
Publication statusPublished - 3 Apr 2019

Fingerprint

ascorbic acid
tomatoes
carbohydrates
fruits
myo-inositol
carbohydrate content
mannose
galactose
after-ripening
nutritional support
pruning
human health
biochemical pathways
biosynthesis
sucrose
flowers
glucose
crops

Cite this

@article{7e63188ee2bb4be19fdacdd5151ee4e9,
title = "Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability",
abstract = "L-ascorbate (ASC) is essential for human health. Therefore, there is interest in increasing the ASC content of crops like tomato. High irradiance induces accumulation of ASC in green tomato fruits. The D-mannose/L-galactose biosynthetic pathway accounts for the most ASC in plants. The myo-inositol and galacturonate pathways have been proposed to exist but never identified in plants. The D-mannose/L-galactose starts from D-glucose. In a series of experiments, we tested the hypothesis that ASC levels depend on soluble carbohydrate content when tomato fruits ripen under irradiances that stimulate ASC biosynthesis. We show that ASC levels considerably increased when fruits ripened under light, but carbohydrate levels did not show a parallel increase. When carbohydrate levels in fruits were altered by flower pruning, no effects onASC levels were observed at harvest or after ripening under irradiances that induce ASC accumulation. Artificial feeding of trusses with sucrose increased carbohydrate levels, but did not affect the light-induced ASC levels. We conclude that light-induced accumulation of ASC is independent of the carbohydrate content in tomato fruits. In tomato fruit treated with light, the increase in ASC was preceded by a concomitant increase in myo-inositol.",
author = "N. Ntagkas and E.J. Woltering and Sofoklis Bouras and {de Vos}, C.H. and J.A. Dieleman and Celine Nicole and C.W. Labrie and L.F.M. Marcelis",
year = "2019",
month = "4",
day = "3",
doi = "10.3390/plants8040086",
language = "English",
volume = "8",
journal = "Plants",
issn = "2223-7747",
publisher = "MDPI AG",
number = "4",

}

Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability. / Ntagkas, N.; Woltering, E.J.; Bouras, Sofoklis; de Vos, C.H.; Dieleman, J.A.; Nicole, Celine; Labrie, C.W.; Marcelis, L.F.M.

In: Plants, Vol. 8, No. 4, 86, 03.04.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Light-Induced Vitamin C Accumulation in Tomato Fruits is Independent of Carbohydrate Availability

AU - Ntagkas, N.

AU - Woltering, E.J.

AU - Bouras, Sofoklis

AU - de Vos, C.H.

AU - Dieleman, J.A.

AU - Nicole, Celine

AU - Labrie, C.W.

AU - Marcelis, L.F.M.

PY - 2019/4/3

Y1 - 2019/4/3

N2 - L-ascorbate (ASC) is essential for human health. Therefore, there is interest in increasing the ASC content of crops like tomato. High irradiance induces accumulation of ASC in green tomato fruits. The D-mannose/L-galactose biosynthetic pathway accounts for the most ASC in plants. The myo-inositol and galacturonate pathways have been proposed to exist but never identified in plants. The D-mannose/L-galactose starts from D-glucose. In a series of experiments, we tested the hypothesis that ASC levels depend on soluble carbohydrate content when tomato fruits ripen under irradiances that stimulate ASC biosynthesis. We show that ASC levels considerably increased when fruits ripened under light, but carbohydrate levels did not show a parallel increase. When carbohydrate levels in fruits were altered by flower pruning, no effects onASC levels were observed at harvest or after ripening under irradiances that induce ASC accumulation. Artificial feeding of trusses with sucrose increased carbohydrate levels, but did not affect the light-induced ASC levels. We conclude that light-induced accumulation of ASC is independent of the carbohydrate content in tomato fruits. In tomato fruit treated with light, the increase in ASC was preceded by a concomitant increase in myo-inositol.

AB - L-ascorbate (ASC) is essential for human health. Therefore, there is interest in increasing the ASC content of crops like tomato. High irradiance induces accumulation of ASC in green tomato fruits. The D-mannose/L-galactose biosynthetic pathway accounts for the most ASC in plants. The myo-inositol and galacturonate pathways have been proposed to exist but never identified in plants. The D-mannose/L-galactose starts from D-glucose. In a series of experiments, we tested the hypothesis that ASC levels depend on soluble carbohydrate content when tomato fruits ripen under irradiances that stimulate ASC biosynthesis. We show that ASC levels considerably increased when fruits ripened under light, but carbohydrate levels did not show a parallel increase. When carbohydrate levels in fruits were altered by flower pruning, no effects onASC levels were observed at harvest or after ripening under irradiances that induce ASC accumulation. Artificial feeding of trusses with sucrose increased carbohydrate levels, but did not affect the light-induced ASC levels. We conclude that light-induced accumulation of ASC is independent of the carbohydrate content in tomato fruits. In tomato fruit treated with light, the increase in ASC was preceded by a concomitant increase in myo-inositol.

U2 - 10.3390/plants8040086

DO - 10.3390/plants8040086

M3 - Article

VL - 8

JO - Plants

T2 - Plants

JF - Plants

SN - 2223-7747

IS - 4

M1 - 86

ER -