Lifespan and the Effects of Dietary Restriction in a Multi-Parent Recombinant Inbred Line Panel in Caenorhabditis elegans

J. Stastna, L.B. Snoek, R.J.M. Volkers, J.A.G. Riksen, J.E. Kammenga, S. Harvey

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Caenorhabditis elegans has been an invaluable model for genetic research for the past few decades. Most C. elegans studies have been carried out using the Bristol N2 isolate, with much natural variation work relying on comparisons between N2 and Hawaiian isolate, CB4856. Recent work has shown that these isolates are genetic outliers, confirming that they are not representative of natural variation within C. elegans. N2 is also highly lab adapted, with many of the quantitative trait loci (QTLs) detected in recombinant inbred lines (RILs) involving N2 resulting from alleles that have arisen in the laboratory. There is therefore a need to consider variation both in other genetic backgrounds and between different groups of isolates. Here, we present an analysis of lifespan under normal conditions (20¿C, NGM plates and ad libitum food) and under conditions of mild dietary restriction (DR) for a new panel of RILs produced from four wild isolates. These strains are representative of genotypically distinct groups of C. elegans isolates from France that differ clearly from both N2 and CB4856. The new panel therefore should be useful in determining the genotype-phenotype relationship that is likely to be happening in the wild. These analyses show there is an extensive variation between the individual lines under both conditions, suggesting polygenic control. We also show extensive variation in the effect of DR on lifespan of the RILs. Here, the majority of the RILs showed the expected response, with DR prolonging lifespan, however in about 1/3 of the RILs DR reduced lifespan. These data are suggestive of either transgressive segregation or of negative epistasis. Future work aims to distinguish between these possible genetic explanations and to relate this variation in lifespan to variation in the stress response.
Original languageEnglish
Title of host publicationProceedings of the Berlin C. elegans Meeting 2014
Pages240-240
Publication statusPublished - 2014
EventBerlin C. elegans Meeting 2014, Berlin, Germany -
Duration: 15 May 201417 May 2014

Conference

ConferenceBerlin C. elegans Meeting 2014, Berlin, Germany
Period15/05/1417/05/14

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