Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men

E.M.P. Backx, A.M.H. Horstman, G.N. Marzuca-Nassr, J. van Kranenburg, J.S. Smeets, C.J. Fuchs, A.A.W. Janssen, C.P.G.M. de Groot, T. Snijders, L.B. Verdijk, L.J.C. van Loon

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Abstract

Background: Short successive periods of physical inactivity occur throughout life and contribute considerably to the age-related loss of skeletal muscle mass. The maintenance of muscle mass during brief periods of disuse is required to prevent functional decline and maintain metabolic health. Objective: To assess whether daily leucine supplementation during a short period of disuse can attenuate subsequent muscle loss in vivo in humans. Methods: Thirty healthy (22 ± 1 y) young males were exposed to a 7-day unilateral knee immobilization intervention by means of a full leg cast with (LEU, n = 15) or without (CON, n = 15) daily leucine supplementation (2.5 g leucine, three times daily). Prior to and directly after immobilization, quadriceps muscle cross-sectional area (computed tomography (CT) scan) and leg strength (one-repetition maximum (1-RM)) were assessed. Furthermore, muscle biopsies were taken in both groups before and after immobilization to assess changes in type I and type II muscle fiber CSA. Results: Quadriceps muscle cross-sectional area (CSA) declined in the CON and LEU groups (p < 0.01), with no differences between the two groups (from 7712 ± 324 to 7287 ± 305 mm2 and from 7643 ± 317 to 7164 ± 328 mm2; p = 0.61, respectively). Leg muscle strength decreased from 56 ± 4 to 53 ± 4 kg in the CON group and from 63 ± 3 to 55 ± 2 kg in the LEU group (main effect of time p < 0.01), with no differences between the groups (p = 0.052). Type I and II muscle fiber size did not change significantly over time, in both groups (p > 0.05). Conclusions: Free leucine supplementation with each of the three main meals (7.5 g/d) does not attenuate the decline of muscle mass and strength during a 7-day limb immobilization intervention.
LanguageEnglish
Article number635
JournalNutrients
Volume10
Issue number5
DOIs
Publication statusPublished - 17 May 2018

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Leucine
Immobilization
leucine
skeletal muscle
Leg
legs
Skeletal Muscle
muscles
Quadriceps Muscle
Muscles
Slow-Twitch Muscle Fibers
Fast-Twitch Muscle Fibers
Muscle Strength
Meals
Knee
Extremities
knees
Tomography
Maintenance
limbs (animal)

Cite this

Backx, E. M. P., Horstman, A. M. H., Marzuca-Nassr, G. N., van Kranenburg, J., Smeets, J. S., Fuchs, C. J., ... van Loon, L. J. C. (2018). Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men. Nutrients, 10(5), [635]. https://doi.org/10.3390/nu10050635
Backx, E.M.P. ; Horstman, A.M.H. ; Marzuca-Nassr, G.N. ; van Kranenburg, J. ; Smeets, J.S. ; Fuchs, C.J. ; Janssen, A.A.W. ; de Groot, C.P.G.M. ; Snijders, T. ; Verdijk, L.B. ; van Loon, L.J.C. / Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men. In: Nutrients. 2018 ; Vol. 10, No. 5.
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title = "Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men",
abstract = "Background: Short successive periods of physical inactivity occur throughout life and contribute considerably to the age-related loss of skeletal muscle mass. The maintenance of muscle mass during brief periods of disuse is required to prevent functional decline and maintain metabolic health. Objective: To assess whether daily leucine supplementation during a short period of disuse can attenuate subsequent muscle loss in vivo in humans. Methods: Thirty healthy (22 ± 1 y) young males were exposed to a 7-day unilateral knee immobilization intervention by means of a full leg cast with (LEU, n = 15) or without (CON, n = 15) daily leucine supplementation (2.5 g leucine, three times daily). Prior to and directly after immobilization, quadriceps muscle cross-sectional area (computed tomography (CT) scan) and leg strength (one-repetition maximum (1-RM)) were assessed. Furthermore, muscle biopsies were taken in both groups before and after immobilization to assess changes in type I and type II muscle fiber CSA. Results: Quadriceps muscle cross-sectional area (CSA) declined in the CON and LEU groups (p < 0.01), with no differences between the two groups (from 7712 ± 324 to 7287 ± 305 mm2 and from 7643 ± 317 to 7164 ± 328 mm2; p = 0.61, respectively). Leg muscle strength decreased from 56 ± 4 to 53 ± 4 kg in the CON group and from 63 ± 3 to 55 ± 2 kg in the LEU group (main effect of time p < 0.01), with no differences between the groups (p = 0.052). Type I and II muscle fiber size did not change significantly over time, in both groups (p > 0.05). Conclusions: Free leucine supplementation with each of the three main meals (7.5 g/d) does not attenuate the decline of muscle mass and strength during a 7-day limb immobilization intervention.",
author = "E.M.P. Backx and A.M.H. Horstman and G.N. Marzuca-Nassr and {van Kranenburg}, J. and J.S. Smeets and C.J. Fuchs and A.A.W. Janssen and {de Groot}, C.P.G.M. and T. Snijders and L.B. Verdijk and {van Loon}, L.J.C.",
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Backx, EMP, Horstman, AMH, Marzuca-Nassr, GN, van Kranenburg, J, Smeets, JS, Fuchs, CJ, Janssen, AAW, de Groot, CPGM, Snijders, T, Verdijk, LB & van Loon, LJC 2018, 'Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men', Nutrients, vol. 10, no. 5, 635. https://doi.org/10.3390/nu10050635

Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men. / Backx, E.M.P.; Horstman, A.M.H.; Marzuca-Nassr, G.N.; van Kranenburg, J.; Smeets, J.S.; Fuchs, C.J.; Janssen, A.A.W.; de Groot, C.P.G.M.; Snijders, T.; Verdijk, L.B.; van Loon, L.J.C.

In: Nutrients, Vol. 10, No. 5, 635, 17.05.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men

AU - Backx, E.M.P.

AU - Horstman, A.M.H.

AU - Marzuca-Nassr, G.N.

AU - van Kranenburg, J.

AU - Smeets, J.S.

AU - Fuchs, C.J.

AU - Janssen, A.A.W.

AU - de Groot, C.P.G.M.

AU - Snijders, T.

AU - Verdijk, L.B.

AU - van Loon, L.J.C.

PY - 2018/5/17

Y1 - 2018/5/17

N2 - Background: Short successive periods of physical inactivity occur throughout life and contribute considerably to the age-related loss of skeletal muscle mass. The maintenance of muscle mass during brief periods of disuse is required to prevent functional decline and maintain metabolic health. Objective: To assess whether daily leucine supplementation during a short period of disuse can attenuate subsequent muscle loss in vivo in humans. Methods: Thirty healthy (22 ± 1 y) young males were exposed to a 7-day unilateral knee immobilization intervention by means of a full leg cast with (LEU, n = 15) or without (CON, n = 15) daily leucine supplementation (2.5 g leucine, three times daily). Prior to and directly after immobilization, quadriceps muscle cross-sectional area (computed tomography (CT) scan) and leg strength (one-repetition maximum (1-RM)) were assessed. Furthermore, muscle biopsies were taken in both groups before and after immobilization to assess changes in type I and type II muscle fiber CSA. Results: Quadriceps muscle cross-sectional area (CSA) declined in the CON and LEU groups (p < 0.01), with no differences between the two groups (from 7712 ± 324 to 7287 ± 305 mm2 and from 7643 ± 317 to 7164 ± 328 mm2; p = 0.61, respectively). Leg muscle strength decreased from 56 ± 4 to 53 ± 4 kg in the CON group and from 63 ± 3 to 55 ± 2 kg in the LEU group (main effect of time p < 0.01), with no differences between the groups (p = 0.052). Type I and II muscle fiber size did not change significantly over time, in both groups (p > 0.05). Conclusions: Free leucine supplementation with each of the three main meals (7.5 g/d) does not attenuate the decline of muscle mass and strength during a 7-day limb immobilization intervention.

AB - Background: Short successive periods of physical inactivity occur throughout life and contribute considerably to the age-related loss of skeletal muscle mass. The maintenance of muscle mass during brief periods of disuse is required to prevent functional decline and maintain metabolic health. Objective: To assess whether daily leucine supplementation during a short period of disuse can attenuate subsequent muscle loss in vivo in humans. Methods: Thirty healthy (22 ± 1 y) young males were exposed to a 7-day unilateral knee immobilization intervention by means of a full leg cast with (LEU, n = 15) or without (CON, n = 15) daily leucine supplementation (2.5 g leucine, three times daily). Prior to and directly after immobilization, quadriceps muscle cross-sectional area (computed tomography (CT) scan) and leg strength (one-repetition maximum (1-RM)) were assessed. Furthermore, muscle biopsies were taken in both groups before and after immobilization to assess changes in type I and type II muscle fiber CSA. Results: Quadriceps muscle cross-sectional area (CSA) declined in the CON and LEU groups (p < 0.01), with no differences between the two groups (from 7712 ± 324 to 7287 ± 305 mm2 and from 7643 ± 317 to 7164 ± 328 mm2; p = 0.61, respectively). Leg muscle strength decreased from 56 ± 4 to 53 ± 4 kg in the CON group and from 63 ± 3 to 55 ± 2 kg in the LEU group (main effect of time p < 0.01), with no differences between the groups (p = 0.052). Type I and II muscle fiber size did not change significantly over time, in both groups (p > 0.05). Conclusions: Free leucine supplementation with each of the three main meals (7.5 g/d) does not attenuate the decline of muscle mass and strength during a 7-day limb immobilization intervention.

U2 - 10.3390/nu10050635

DO - 10.3390/nu10050635

M3 - Article

VL - 10

JO - Nutrients

T2 - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 5

M1 - 635

ER -

Backx EMP, Horstman AMH, Marzuca-Nassr GN, van Kranenburg J, Smeets JS, Fuchs CJ et al. Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men. Nutrients. 2018 May 17;10(5). 635. https://doi.org/10.3390/nu10050635