Legacy effects on the recovery of soil bacterial communities from extreme temperature perturbation

Stephanie D. Jurburg*, Inês Nunes, Asker Brejnrod, Samuel Jacquiod, Anders Priemé, Søren J. Sørensen, J.D. van Elsas, Joana F. Salles

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

The type and frequency of disturbances experienced by soil microbiomes is expected to increase given predicted global climate change scenarios and intensified anthropogenic pressures on ecosystems. While the direct effect of multiple disturbances to soil microbes has been explored in terms of function, their effect on the recovery of microbial community composition remains unclear. Here, we used soil microcosm experiments and multiple model disturbances to explore their short-term effect on the recovery of soil microbiota after identical or novel stresses. Soil microcosms were exposed to a heat shock to create an initial effect. Upon initial community recovery (25 days after stress), they were subjected to a second stress, either a heat or a cold shock, and they were monitored for additional 25 days. To carefully verify the bacterial response to the disturbances, we monitored changes in community composition throughout the experiment using 16S rRNA gene transcript amplicon sequencing. The application of a heat shock to soils with or without the initial heat shock resulted in similar successional dynamics, but these dynamics were faster in soils with a prior heat shock. The application of a cold shock had negligible effects on previously undisturbed soils but, in combination with an initial heat shock, caused the largest shift in the community composition. Our findings show that compounded perturbation affects bacterial community recovery by altering community structure and thus, the community's response during succession. By altering dominance patterns, disturbance legacy affects the microbiome's ability to recover from further perturbation within the 25 days studied. Our results highlight the need to consider the soil's disturbance history in the development of soil management practices in order to maintain the system's resilience.

Original languageEnglish
Article number1832
Number of pages13
JournalFrontiers in Microbiology
Volume8
DOIs
Publication statusPublished - 25 Sep 2017

Fingerprint

Soil
Temperature
Shock
Hot Temperature
Microbiota
Climate Change
Practice Management
rRNA Genes
Ecosystem
History
Pressure

Keywords

  • Disturbance
  • Microcosm
  • Resilience
  • RNA
  • Secondary succession
  • Soil bacteria

Cite this

Jurburg, S. D., Nunes, I., Brejnrod, A., Jacquiod, S., Priemé, A., Sørensen, S. J., ... Salles, J. F. (2017). Legacy effects on the recovery of soil bacterial communities from extreme temperature perturbation. Frontiers in Microbiology, 8, [1832]. https://doi.org/10.3389/fmicb.2017.01832
Jurburg, Stephanie D. ; Nunes, Inês ; Brejnrod, Asker ; Jacquiod, Samuel ; Priemé, Anders ; Sørensen, Søren J. ; van Elsas, J.D. ; Salles, Joana F. / Legacy effects on the recovery of soil bacterial communities from extreme temperature perturbation. In: Frontiers in Microbiology. 2017 ; Vol. 8.
@article{d2ec341c03dd4d9f85a422bfbd65feb5,
title = "Legacy effects on the recovery of soil bacterial communities from extreme temperature perturbation",
abstract = "The type and frequency of disturbances experienced by soil microbiomes is expected to increase given predicted global climate change scenarios and intensified anthropogenic pressures on ecosystems. While the direct effect of multiple disturbances to soil microbes has been explored in terms of function, their effect on the recovery of microbial community composition remains unclear. Here, we used soil microcosm experiments and multiple model disturbances to explore their short-term effect on the recovery of soil microbiota after identical or novel stresses. Soil microcosms were exposed to a heat shock to create an initial effect. Upon initial community recovery (25 days after stress), they were subjected to a second stress, either a heat or a cold shock, and they were monitored for additional 25 days. To carefully verify the bacterial response to the disturbances, we monitored changes in community composition throughout the experiment using 16S rRNA gene transcript amplicon sequencing. The application of a heat shock to soils with or without the initial heat shock resulted in similar successional dynamics, but these dynamics were faster in soils with a prior heat shock. The application of a cold shock had negligible effects on previously undisturbed soils but, in combination with an initial heat shock, caused the largest shift in the community composition. Our findings show that compounded perturbation affects bacterial community recovery by altering community structure and thus, the community's response during succession. By altering dominance patterns, disturbance legacy affects the microbiome's ability to recover from further perturbation within the 25 days studied. Our results highlight the need to consider the soil's disturbance history in the development of soil management practices in order to maintain the system's resilience.",
keywords = "Disturbance, Microcosm, Resilience, RNA, Secondary succession, Soil bacteria",
author = "Jurburg, {Stephanie D.} and In{\^e}s Nunes and Asker Brejnrod and Samuel Jacquiod and Anders Priem{\'e} and S{\o}rensen, {S{\o}ren J.} and {van Elsas}, J.D. and Salles, {Joana F.}",
year = "2017",
month = "9",
day = "25",
doi = "10.3389/fmicb.2017.01832",
language = "English",
volume = "8",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers",

}

Jurburg, SD, Nunes, I, Brejnrod, A, Jacquiod, S, Priemé, A, Sørensen, SJ, van Elsas, JD & Salles, JF 2017, 'Legacy effects on the recovery of soil bacterial communities from extreme temperature perturbation', Frontiers in Microbiology, vol. 8, 1832. https://doi.org/10.3389/fmicb.2017.01832

Legacy effects on the recovery of soil bacterial communities from extreme temperature perturbation. / Jurburg, Stephanie D.; Nunes, Inês; Brejnrod, Asker; Jacquiod, Samuel; Priemé, Anders; Sørensen, Søren J.; van Elsas, J.D.; Salles, Joana F.

In: Frontiers in Microbiology, Vol. 8, 1832, 25.09.2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Legacy effects on the recovery of soil bacterial communities from extreme temperature perturbation

AU - Jurburg, Stephanie D.

AU - Nunes, Inês

AU - Brejnrod, Asker

AU - Jacquiod, Samuel

AU - Priemé, Anders

AU - Sørensen, Søren J.

AU - van Elsas, J.D.

AU - Salles, Joana F.

PY - 2017/9/25

Y1 - 2017/9/25

N2 - The type and frequency of disturbances experienced by soil microbiomes is expected to increase given predicted global climate change scenarios and intensified anthropogenic pressures on ecosystems. While the direct effect of multiple disturbances to soil microbes has been explored in terms of function, their effect on the recovery of microbial community composition remains unclear. Here, we used soil microcosm experiments and multiple model disturbances to explore their short-term effect on the recovery of soil microbiota after identical or novel stresses. Soil microcosms were exposed to a heat shock to create an initial effect. Upon initial community recovery (25 days after stress), they were subjected to a second stress, either a heat or a cold shock, and they were monitored for additional 25 days. To carefully verify the bacterial response to the disturbances, we monitored changes in community composition throughout the experiment using 16S rRNA gene transcript amplicon sequencing. The application of a heat shock to soils with or without the initial heat shock resulted in similar successional dynamics, but these dynamics were faster in soils with a prior heat shock. The application of a cold shock had negligible effects on previously undisturbed soils but, in combination with an initial heat shock, caused the largest shift in the community composition. Our findings show that compounded perturbation affects bacterial community recovery by altering community structure and thus, the community's response during succession. By altering dominance patterns, disturbance legacy affects the microbiome's ability to recover from further perturbation within the 25 days studied. Our results highlight the need to consider the soil's disturbance history in the development of soil management practices in order to maintain the system's resilience.

AB - The type and frequency of disturbances experienced by soil microbiomes is expected to increase given predicted global climate change scenarios and intensified anthropogenic pressures on ecosystems. While the direct effect of multiple disturbances to soil microbes has been explored in terms of function, their effect on the recovery of microbial community composition remains unclear. Here, we used soil microcosm experiments and multiple model disturbances to explore their short-term effect on the recovery of soil microbiota after identical or novel stresses. Soil microcosms were exposed to a heat shock to create an initial effect. Upon initial community recovery (25 days after stress), they were subjected to a second stress, either a heat or a cold shock, and they were monitored for additional 25 days. To carefully verify the bacterial response to the disturbances, we monitored changes in community composition throughout the experiment using 16S rRNA gene transcript amplicon sequencing. The application of a heat shock to soils with or without the initial heat shock resulted in similar successional dynamics, but these dynamics were faster in soils with a prior heat shock. The application of a cold shock had negligible effects on previously undisturbed soils but, in combination with an initial heat shock, caused the largest shift in the community composition. Our findings show that compounded perturbation affects bacterial community recovery by altering community structure and thus, the community's response during succession. By altering dominance patterns, disturbance legacy affects the microbiome's ability to recover from further perturbation within the 25 days studied. Our results highlight the need to consider the soil's disturbance history in the development of soil management practices in order to maintain the system's resilience.

KW - Disturbance

KW - Microcosm

KW - Resilience

KW - RNA

KW - Secondary succession

KW - Soil bacteria

U2 - 10.3389/fmicb.2017.01832

DO - 10.3389/fmicb.2017.01832

M3 - Article

VL - 8

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 1832

ER -