Abstract
Plant breeders continuously face the challenge to obtain new cultivars with adequate
levels of resistance to a variety of pathogens. In recent years, we identified a novel type
of immune receptors that could be employed as disease resistance components
towards both Phytophthora and bacterial pathogens. This resistance is mediated by cell
surface receptors belonging to the family of L-type lectin receptor kinases (LecRKs).
LecRKs are wide-spread in plants, and justifies exploitation of LecRKs as novel sources
of crop resistance. The LecRK multi-gene family in Arabidopsis consists of 45 members,
and their individual role in defense was determined in a genome-wide phenotypic
analysis of T-DNA insertion mutants in infection assays. We found that multiple LecRKs
play a role in resistance to a variety of plant pathogens, and that overexpression of
various LecRKs enhances disease resistance. In addition, we screened for LecRKlnteracting-
Proteins (LIPs) using mass spectrometry. LecRK interaction was confirmed
for one of the candidates, LIP1, in co-immunoprecipitation assays. Further functional
analysis showed that Arabidopsis Lip1 mutants are compromised in Phytophthora
resistance in a similar fashion as LecRK mutant lines. Understanding how LecRKmediated
resistance is functioning is crucial to design novel resistance in crops against
Phytophthora and bacterial plant pathogens.
| Original language | English |
|---|---|
| Title of host publication | Book of Abstracts Oomycete Molecular Genetics Network Meeting |
| Pages | 17 |
| Publication status | Published - 2015 |
| Event | Oomycete Molecular Genetics Network Meeting 2015 - Pacific Grove, United States Duration: 14 Mar 2015 → 17 Mar 2015 |
Conference/symposium
| Conference/symposium | Oomycete Molecular Genetics Network Meeting 2015 |
|---|---|
| Country/Territory | United States |
| City | Pacific Grove |
| Period | 14/03/15 → 17/03/15 |