LecRK79, a putative virulence target of the RXLR effector IPI-O is involved in cell wall-plasma membrane adhesions and PAMP-triggered immunity

M. de Sain, K. Bouwmeester, R. Weide, H. Canut, F. Govers

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

The potato late blight pathogen Phytophthora infestans secretes many RXLR effectors, one of which is IPI-O. In IPI-O the RXLR motif overlaps with the tripeptide RGD (RSLRGD), a typical cell adhesion motif present in extracellular metazoan proteins that play a role in cell-cell interactions. A phage display aimed at selecting proteins that potentially interact with the RGD motif in IPI-O resulted in the identification of Arabidopsis lectin receptor kinase LecRK79 (Gouget et al. 2006, Plant Physiology 140, 81-90). We postulate that LecRK79 is a virulence target of IPI-O. For functional characterization we generated Arabidopsis lines overexpressing LecRK79 (OE lines) or lacking LecRK79 (knock-out lines lecrk79). Infection assays revealed changes in phenotype upon infection with Phytophthora brassicae in both the OE and lecrk79 lines and demonstrate that LecRK79 has a role in Phytophthora disease resistance. To unravel the mechanisms underlying this resistance we analysed callose deposition upon PAMP treatment and investigated the strength of cell wall-plasma membrane adhesions by inducing plasmolysis. The results indicate that LecRK79 plays an important role in the continuum between cell wall and plasma membrane and suggest that this lectin receptor kinase is involved in PAMP-triggered immunity
Original languageEnglish
Title of host publicationBook of Abstracts 22nd New Phytologist Symposium, Paris, France, 13-16 September 2009
Pages40
Publication statusPublished - 2009
Event22nd New Phytologist Symposium, Paris, France -
Duration: 13 Sep 200916 Sep 2009

Conference

Conference22nd New Phytologist Symposium, Paris, France
Period13/09/0916/09/09

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    de Sain, M., Bouwmeester, K., Weide, R., Canut, H., & Govers, F. (2009). LecRK79, a putative virulence target of the RXLR effector IPI-O is involved in cell wall-plasma membrane adhesions and PAMP-triggered immunity. In Book of Abstracts 22nd New Phytologist Symposium, Paris, France, 13-16 September 2009 (pp. 40)