Laser ablation electrospray ionization-mass spectrometry imaging (LAESI-MS) for spatially resolved plant metabolomics

Desalegn W. Etalo, Carmen Díez-Simón, Ric C.H. de Vos, Robert D. Hall*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

There is increasing demand to bring the level of metabolomics analyses down to the tissue or cell level. Significant progress has been made involving the use of in situ metabolomics imaging techniques where no tissue collection or extraction is needed prior to analysis. In this chapter we describe a relatively new method which is simple and easy to use. No ectopic matrix or vacuum is required, and analyses are performed with living plant materials directly from (or even still attached to) the plant. Although relatively straightforward, there are still a few caveats as regards this method which are described at the end of the chapter.
Original languageEnglish
Title of host publicationPlant Metabolomics
Subtitle of host publicationMethods and Protocols
EditorsCarla António
Place of PublicationNew York
PublisherHumana Press Inc.
Chapter18
Pages253-267
ISBN (Electronic)9781493978199
ISBN (Print)9781493978182
DOIs
Publication statusPublished - 15 May 2018

Publication series

NameMethods in Molecular Biology
Volume1778
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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Keywords

  • Imaging
  • In situ metabolomics
  • LAESI
  • Laser ablation ESI-MS
  • Plant metabolomics
  • Spatial resolution

Cite this

Etalo, D. W., Díez-Simón, C., de Vos, R. C. H., & Hall, R. D. (2018). Laser ablation electrospray ionization-mass spectrometry imaging (LAESI-MS) for spatially resolved plant metabolomics. In C. António (Ed.), Plant Metabolomics: Methods and Protocols (pp. 253-267). (Methods in Molecular Biology; Vol. 1778). New York: Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7819-9_18