Lachnotalea glycerini gen. nov., sp. nov., a novel anaerobe isolated from a nanofiltration unit treating anoxic groundwater

M. Jarzembowska, D.Z. Sousa, F. Beyer, A. Zwijnenburg, C.M. Plugge, A.J.M. Stams

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

A strictly anaerobic bacterium, strain DLD10T, was isolated from a biofilm that developed on a nanofiltration membrane treating anoxic groundwater using glycerol as substrate. Cells were straight to slightly curved rods 0.2 - 0.5 µm in diameter and 1 - 3 µm in length, non-motile and non-spore forming. The optimum temperature and pH for growth were 30˚C and pH 7.0, respectively. Strain DLD10T could grow in the presence of 0.03 - 4.5% NaCl (w/v). Substrates utilised by strain DLD10T included glycerol and various carbohydrates (glucose, sucrose, fructose, mannose, arabinose, pectin, starch, xylan), which were mainly converted to ethanol, acetate, H2 and formate. Thiosulphate, sulphur and Fe (III) were used as electron acceptors, but sulphate, fumarate and nitrate not. The predominant membrane fatty acids were C16:0, iso-C17:1 and C17:1 ω8c. The DNA G + C content was 36.4 mol %. Strain DLD10T belongs to the family Lachnospiraceae and is distantly related to Clostridium populeti DSM 5832T, Hespellia porcina DSM 15481T, and Robinsoniella peoriensis CCUG 48729T (93% 16S rRNA gene identity). Physiological characteristics and phylogenetic analysis revealed that strain DLD10T is a representative of a novel species of a new genus, for which the name Lachnotalea glycerini gen. nov., sp. nov., is proposed. The type strain of Lachnotalea glycerini is DLD10T (=DSM 28816T = JCM 30818T)
Original languageEnglish
Pages (from-to)774-779
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number2
DOIs
Publication statusPublished - 2016

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