Twenty three accessions of L. peruvianum were screened for resistance to Clavibater michiganensis ssp. michiganensis (bacterial canker). Resistance was detected in five accessions. One of these, L. peruvianum LA2157 was crossed with a susceptible accession of L. peruvainum and with the cultivated tomato. Evaluation of the segragation of the progenies for disease resistance and of RFLP markers identified several regions that are involved bacterial canker resistance. Two resistance loci cross explained almost completely the difference in resistance between L. esculantum and L. peruvianum LA2157 and offers good potentials for breeding tomato for bacterial canker resistance.