Kunjin replicon-based simian immunodeficiency virus gag vaccines

I. Anruka; V. Mokhonov; P. Rattanasena; E. Mokhonova; J.Y. Leung; G.P. Pijlman; A. Cara; W.A. Schroder; A.A. Khromykh; A. Suhrbier

doi:10.1016/j.vaccine.2008.04.001

Kunjin replicon-based simian immunodeficiency virus gag vaccines

I. Anruka, V. Mokhonov, P. Rattanasena, E. Mokhonova, J.Y. Leung, G.P. Pijlman, A. Cara, W.A. Schroder, A.A. Khromykh, A. Suhrbier

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

An RNA-based, non-cytopathic replicon vector system, based on the flavivirus Kunjin, has shown considerable promise as a new vaccine delivery system. Here we describe the testing in mice of four different SIVmac239 gag vaccines delivered by Kunjin replicon virus-like-particles. The four vaccines encoded the wild type gag gene, an RNA-optimised gag gene, a codon-optimised gag gene and a modified gag-pol gene construct. The vaccines behaved quite differently for induction of effector memory and central memory responses, for mediation of protection, and with respect to insert stability, with the SIV gag-pol vaccine providing the optimal performance. These results illustrate that for an RNA-based vector the RNA sequence of the antigen can have profound and unforeseen consequences on vaccine behaviour.

Original language	English
Pages (from-to)	3268-3276
Journal	Vaccine
Volume	26
Issue number	26
DOIs	https://doi.org/10.1016/j.vaccine.2008.04.001
Publication status	Published - 2008

Keywords

cellular immune-responses
expressing hiv-1 gag
cd8(+) t-cells
gene-therapy
recombinant adenovirus
infected-cells
sindbis-virus
vector system
in-vivo
protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2008.04.001

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title = "Kunjin replicon-based simian immunodeficiency virus gag vaccines",

abstract = "An RNA-based, non-cytopathic replicon vector system, based on the flavivirus Kunjin, has shown considerable promise as a new vaccine delivery system. Here we describe the testing in mice of four different SIVmac239 gag vaccines delivered by Kunjin replicon virus-like-particles. The four vaccines encoded the wild type gag gene, an RNA-optimised gag gene, a codon-optimised gag gene and a modified gag-pol gene construct. The vaccines behaved quite differently for induction of effector memory and central memory responses, for mediation of protection, and with respect to insert stability, with the SIV gag-pol vaccine providing the optimal performance. These results illustrate that for an RNA-based vector the RNA sequence of the antigen can have profound and unforeseen consequences on vaccine behaviour.",

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author = "I. Anruka and V. Mokhonov and P. Rattanasena and E. Mokhonova and J.Y. Leung and G.P. Pijlman and A. Cara and W.A. Schroder and A.A. Khromykh and A. Suhrbier",

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doi = "10.1016/j.vaccine.2008.04.001",

language = "English",

volume = "26",

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T1 - Kunjin replicon-based simian immunodeficiency virus gag vaccines

AU - Anruka, I.

AU - Mokhonov, V.

AU - Rattanasena, P.

AU - Mokhonova, E.

AU - Leung, J.Y.

AU - Pijlman, G.P.

AU - Cara, A.

AU - Schroder, W.A.

AU - Khromykh, A.A.

AU - Suhrbier, A.

PY - 2008

Y1 - 2008

N2 - An RNA-based, non-cytopathic replicon vector system, based on the flavivirus Kunjin, has shown considerable promise as a new vaccine delivery system. Here we describe the testing in mice of four different SIVmac239 gag vaccines delivered by Kunjin replicon virus-like-particles. The four vaccines encoded the wild type gag gene, an RNA-optimised gag gene, a codon-optimised gag gene and a modified gag-pol gene construct. The vaccines behaved quite differently for induction of effector memory and central memory responses, for mediation of protection, and with respect to insert stability, with the SIV gag-pol vaccine providing the optimal performance. These results illustrate that for an RNA-based vector the RNA sequence of the antigen can have profound and unforeseen consequences on vaccine behaviour.

AB - An RNA-based, non-cytopathic replicon vector system, based on the flavivirus Kunjin, has shown considerable promise as a new vaccine delivery system. Here we describe the testing in mice of four different SIVmac239 gag vaccines delivered by Kunjin replicon virus-like-particles. The four vaccines encoded the wild type gag gene, an RNA-optimised gag gene, a codon-optimised gag gene and a modified gag-pol gene construct. The vaccines behaved quite differently for induction of effector memory and central memory responses, for mediation of protection, and with respect to insert stability, with the SIV gag-pol vaccine providing the optimal performance. These results illustrate that for an RNA-based vector the RNA sequence of the antigen can have profound and unforeseen consequences on vaccine behaviour.

KW - cellular immune-responses

KW - expressing hiv-1 gag

KW - cd8(+) t-cells

KW - gene-therapy

KW - recombinant adenovirus

KW - infected-cells

KW - sindbis-virus

KW - vector system

KW - in-vivo

KW - protein

U2 - 10.1016/j.vaccine.2008.04.001

DO - 10.1016/j.vaccine.2008.04.001

M3 - Article

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VL - 26

SP - 3268

EP - 3276

JO - Vaccine

JF - Vaccine

IS - 26

ER -

Kunjin replicon-based simian immunodeficiency virus gag vaccines

Abstract

Keywords

UN SDGs

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