KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery

N. Mansoori Zangir, J.F.P. Timmers, T. Desprez, C. Lessa Alvim Kamei, D.C.T. Dees, J.P. Vincken, R.G.F. Visser, H. Höfte, S. Vernhettes, L.M. Trindade

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

Cellulose is synthesized by the so called rosette protein complex and the catalytic subunits of this complex are the cellulose synthases (CESAs). It is thought that the rosette complexes in the primary and secondary cell walls each contains at least three different non-redundant cellulose synthases. In addition to the CESA proteins, cellulose biosynthesis almost certainly requires the action of other proteins, although few have been identified and little is known about the biochemical role of those that have been identified. One of these proteins is KORRIGAN (KOR1). Mutant analysis of this protein in Arabidopsis thaliana showed altered cellulose content in both the primary and secondary cell wall. KOR1 is thought to be required for cellulose synthesis acting as a cellulase at the plasma membrane–cell wall interface. KOR1 has recently been shown to interact with the primary cellulose synthase rosette complex however direct interaction with that of the secondary cell wall has never been demonstrated. Using various methods, both in vitro and in planta, it was shown that KOR1 interacts specifically with only two of the secondary CESA proteins. The KOR1 protein domain(s) involved in the interaction with the CESA proteins were also identified by analyzing the interaction of truncated forms of KOR1 with CESA proteins. The KOR1 transmembrane domain has shown to be required for the interaction between KOR1 and the different CESAs, as well as for higher oligomer formation of KOR1.
Original languageEnglish
Article numbere112387
JournalPLoS ONE
Volume9
Issue number11
DOIs
Publication statusPublished - 2014

Fingerprint

cellulose synthase
Machinery
Cellulose
Proteins
proteins
cellulose
Cell Wall
Cells
cell walls
Cellulase
Biosynthesis
Protein Biosynthesis
Mutant Proteins
Oligomers
Arabidopsis
protein subunits
Plantae
endo-1,4-beta-glucanase
Catalytic Domain
Arabidopsis thaliana

Keywords

  • secondary cell-wall
  • arabidopsis-thaliana
  • endo-1,4-beta-glucanase
  • expression
  • membranes
  • protein
  • system
  • plants
  • gene
  • endo-1,4-beta-d-glucanase

Cite this

Mansoori Zangir, N. ; Timmers, J.F.P. ; Desprez, T. ; Lessa Alvim Kamei, C. ; Dees, D.C.T. ; Vincken, J.P. ; Visser, R.G.F. ; Höfte, H. ; Vernhettes, S. ; Trindade, L.M. / KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery. In: PLoS ONE. 2014 ; Vol. 9, No. 11.
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title = "KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery",
abstract = "Cellulose is synthesized by the so called rosette protein complex and the catalytic subunits of this complex are the cellulose synthases (CESAs). It is thought that the rosette complexes in the primary and secondary cell walls each contains at least three different non-redundant cellulose synthases. In addition to the CESA proteins, cellulose biosynthesis almost certainly requires the action of other proteins, although few have been identified and little is known about the biochemical role of those that have been identified. One of these proteins is KORRIGAN (KOR1). Mutant analysis of this protein in Arabidopsis thaliana showed altered cellulose content in both the primary and secondary cell wall. KOR1 is thought to be required for cellulose synthesis acting as a cellulase at the plasma membrane–cell wall interface. KOR1 has recently been shown to interact with the primary cellulose synthase rosette complex however direct interaction with that of the secondary cell wall has never been demonstrated. Using various methods, both in vitro and in planta, it was shown that KOR1 interacts specifically with only two of the secondary CESA proteins. The KOR1 protein domain(s) involved in the interaction with the CESA proteins were also identified by analyzing the interaction of truncated forms of KOR1 with CESA proteins. The KOR1 transmembrane domain has shown to be required for the interaction between KOR1 and the different CESAs, as well as for higher oligomer formation of KOR1.",
keywords = "secondary cell-wall, arabidopsis-thaliana, endo-1,4-beta-glucanase, expression, membranes, protein, system, plants, gene, endo-1,4-beta-d-glucanase",
author = "{Mansoori Zangir}, N. and J.F.P. Timmers and T. Desprez and {Lessa Alvim Kamei}, C. and D.C.T. Dees and J.P. Vincken and R.G.F. Visser and H. H{\"o}fte and S. Vernhettes and L.M. Trindade",
year = "2014",
doi = "10.1371/journal.pone.0112387",
language = "English",
volume = "9",
journal = "PLoS ONE",
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KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery. / Mansoori Zangir, N.; Timmers, J.F.P.; Desprez, T.; Lessa Alvim Kamei, C.; Dees, D.C.T.; Vincken, J.P.; Visser, R.G.F.; Höfte, H.; Vernhettes, S.; Trindade, L.M.

In: PLoS ONE, Vol. 9, No. 11, e112387, 2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery

AU - Mansoori Zangir, N.

AU - Timmers, J.F.P.

AU - Desprez, T.

AU - Lessa Alvim Kamei, C.

AU - Dees, D.C.T.

AU - Vincken, J.P.

AU - Visser, R.G.F.

AU - Höfte, H.

AU - Vernhettes, S.

AU - Trindade, L.M.

PY - 2014

Y1 - 2014

N2 - Cellulose is synthesized by the so called rosette protein complex and the catalytic subunits of this complex are the cellulose synthases (CESAs). It is thought that the rosette complexes in the primary and secondary cell walls each contains at least three different non-redundant cellulose synthases. In addition to the CESA proteins, cellulose biosynthesis almost certainly requires the action of other proteins, although few have been identified and little is known about the biochemical role of those that have been identified. One of these proteins is KORRIGAN (KOR1). Mutant analysis of this protein in Arabidopsis thaliana showed altered cellulose content in both the primary and secondary cell wall. KOR1 is thought to be required for cellulose synthesis acting as a cellulase at the plasma membrane–cell wall interface. KOR1 has recently been shown to interact with the primary cellulose synthase rosette complex however direct interaction with that of the secondary cell wall has never been demonstrated. Using various methods, both in vitro and in planta, it was shown that KOR1 interacts specifically with only two of the secondary CESA proteins. The KOR1 protein domain(s) involved in the interaction with the CESA proteins were also identified by analyzing the interaction of truncated forms of KOR1 with CESA proteins. The KOR1 transmembrane domain has shown to be required for the interaction between KOR1 and the different CESAs, as well as for higher oligomer formation of KOR1.

AB - Cellulose is synthesized by the so called rosette protein complex and the catalytic subunits of this complex are the cellulose synthases (CESAs). It is thought that the rosette complexes in the primary and secondary cell walls each contains at least three different non-redundant cellulose synthases. In addition to the CESA proteins, cellulose biosynthesis almost certainly requires the action of other proteins, although few have been identified and little is known about the biochemical role of those that have been identified. One of these proteins is KORRIGAN (KOR1). Mutant analysis of this protein in Arabidopsis thaliana showed altered cellulose content in both the primary and secondary cell wall. KOR1 is thought to be required for cellulose synthesis acting as a cellulase at the plasma membrane–cell wall interface. KOR1 has recently been shown to interact with the primary cellulose synthase rosette complex however direct interaction with that of the secondary cell wall has never been demonstrated. Using various methods, both in vitro and in planta, it was shown that KOR1 interacts specifically with only two of the secondary CESA proteins. The KOR1 protein domain(s) involved in the interaction with the CESA proteins were also identified by analyzing the interaction of truncated forms of KOR1 with CESA proteins. The KOR1 transmembrane domain has shown to be required for the interaction between KOR1 and the different CESAs, as well as for higher oligomer formation of KOR1.

KW - secondary cell-wall

KW - arabidopsis-thaliana

KW - endo-1,4-beta-glucanase

KW - expression

KW - membranes

KW - protein

KW - system

KW - plants

KW - gene

KW - endo-1,4-beta-d-glucanase

U2 - 10.1371/journal.pone.0112387

DO - 10.1371/journal.pone.0112387

M3 - Article

VL - 9

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 11

M1 - e112387

ER -