Kinetics of antiviral CD8 T cell responses during primary and post-vaccination secondary bovine respiratory syncytial virus infection

A.F.G. Antonis, E.A.W. Claassen, E.J. Hensen, R.J. de Groot, J.D.F. de Groot-Mijnes, R.S. Schrijver, R.G. van der Most

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    22 Citations (Scopus)

    Abstract

    We have measured antiviral CD8 T cells responses in bovine respiratory syncytial virus (bRSV) infected calves that had been immunized with either formalin-inactivated (FI) or live-attenuated (L) bRSV, with evidence of immunopathology following challenge of calves vaccinated with FI-bRSV. In all cases, bRSV infection induced potent pulmonary CD8 T cell responses. The kinetics of the post-challenge response in L-bRSV immunized animals was accelerated compared to the FI-bRSV and PBS groups, suggesting that only the L-bRSV vaccine, and not the FI-bRSV vaccine, had primed memory T cells. The differences between primary and post-vaccination secondary infection were very minor, in terms of the proliferation status of pulmonary CD8 T cells. Functional IFN-¿+ CD8 responses were slightly higher in the FI-bRSV vaccinated animals. Furthermore, the existence of strong IFN-¿+ CD8 responses in FI-bRSV vaccinated animals after challenge suggests (i) that these IFN-¿+ responses in FI-bRSV immunized animals do not protect against immunopathology, and (ii) that Th-2 biased responses during bRSV challenge after vaccination with FI-bRSV have a limited impact on the CD8 responses in the bronchoalveolar lavage fluid. Thus, several response patterns (Th-l/Th-2) seem to co-exist during bRSV infection
    Original languageEnglish
    Pages (from-to)1551-1561
    JournalVaccine
    Volume24
    Issue number10
    DOIs
    Publication statusPublished - 2006

    Keywords

    • recombinant vaccinia virus
    • formalin-inactivated rsv
    • immune-response
    • g-glycoprotein
    • induced immunopathology
    • lymphocyte activity
    • lung pathology
    • ifn-gamma
    • calves
    • disease

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