Key steps towards the oriented immobilization of antibodies using boronic acids

F.L. Duval, T.A. van Beek*, H. Zuilhof

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

50 Citations (Scopus)

Abstract

Oriented immobilization of antibodies using boronic acids shows a strong potential for improving immunoassay performance but is not yet widely used, possibly because of the difficulties encountered in its implementation. How to choose the boronic acid structure and how should it be attached to the surface? How to choose an antibody that will bind to the boronic acid? Under which conditions should the binding take place for an effective oriented antibody immobilization? How to make sure that the antibody stays on the surface? This tutorial review provides answers to these questions through analysis of the literature and personal suggestions, and thereby intends to facilitate the development of this promising antibody immobilization strategy.
Original languageEnglish
Pages (from-to)6467-6472
JournalThe Analyst
Volume140
Issue number19
DOIs
Publication statusPublished - 2015

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