Karyotyping Methods for Fungi

R. Mehrabi, M. Taga, M. Aghaee, P.J.G.M. de Wit, G.H.J. Kema

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

6 Citations (Scopus)


Pulsed fi eld gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes
Original languageEnglish
Title of host publicationPlant Fungal Pathogens : Methods and Protocols
EditorsM.D.D. Bolton, B.P.H.J.P. Thomma
Place of PublicationNew York, Dordrecht, Heidelberg, London
Number of pages645
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology


  • Chromosome polymorphisms
  • Electrophoretic karyotyping
  • Germ tube burst method
  • Pulsed field gel electrophoresis


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