Karyotyping Methods for Fungi

R. Mehrabi; M. Taga; M. Aghaee; P.J.G.M. de Wit; G.H.J. Kema

doi:10.1007/978-1-61779-501-5_37

Karyotyping Methods for Fungi

R. Mehrabi, M. Taga, M. Aghaee, P.J.G.M. de Wit, G.H.J. Kema

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

6 Citations (Scopus)

Abstract

Pulsed fi eld gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes

Original language	English
Title of host publication	Plant Fungal Pathogens : Methods and Protocols
Editors	M.D.D. Bolton, B.P.H.J.P. Thomma
Place of Publication	New York, Dordrecht, Heidelberg, London
Pages	591-602
Number of pages	645
DOIs	https://doi.org/10.1007/978-1-61779-501-5_37
Publication status	Published - 2012

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Number	835

Keywords

Chromosome polymorphisms
Electrophoretic karyotyping
Germ tube burst method
Pulsed field gel electrophoresis

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10.1007/978-1-61779-501-5_37

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abstract = "Pulsed fi eld gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes",

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Karyotyping Methods for Fungi. / Mehrabi, R.; Taga, M.; Aghaee, M.; de Wit, P.J.G.M.; Kema, G.H.J.

Plant Fungal Pathogens : Methods and Protocols. ed. / M.D.D. Bolton; B.P.H.J.P. Thomma. New York, Dordrecht, Heidelberg, London, 2012. p. 591-602 (Methods in Molecular Biology; No. 835).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

TY - CHAP

T1 - Karyotyping Methods for Fungi

AU - Mehrabi, R.

AU - Taga, M.

AU - Aghaee, M.

AU - de Wit, P.J.G.M.

AU - Kema, G.H.J.

PY - 2012

Y1 - 2012

N2 - Pulsed fi eld gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes

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KW - Chromosome polymorphisms

KW - Electrophoretic karyotyping

KW - Germ tube burst method

KW - Pulsed field gel electrophoresis

U2 - 10.1007/978-1-61779-501-5_37

DO - 10.1007/978-1-61779-501-5_37

M3 - Chapter

SN - 9781617795008

T3 - Methods in Molecular Biology

SP - 591

EP - 602

BT - Plant Fungal Pathogens : Methods and Protocols

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A2 - Thomma, B.P.H.J.P.

CY - New York, Dordrecht, Heidelberg, London

ER -

Karyotyping Methods for Fungi

Abstract

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Keywords

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