Isolation of recombinant antibodies (scFvs) to grapevine virus B

P. Saldarelli; H.J.H.G. Keller; M. Dell'Orco; A. Schots; V. Elicio; A. Minafra

doi:10.1016/j.jviromet.2004.11.021

Isolation of recombinant antibodies (scFvs) to grapevine virus B

P. Saldarelli, H.J.H.G. Keller, M. Dell'Orco, A. Schots, V. Elicio, A. Minafra

Laboratory of Nematology

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

Abstract

A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.

Original language	English
Pages (from-to)	191-195
Journal	Journal of Virological Methods
Volume	124
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2004.11.021
Publication status	Published - 2005

Keywords

phage display libraries
fusion proteins
diagnosis
reagents
elisa

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10.1016/j.jviromet.2004.11.021

https://edepot.wur.nl/44089Licence: Taverne

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title = "Isolation of recombinant antibodies (scFvs) to grapevine virus B",

abstract = "A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.",

keywords = "phage display libraries, fusion proteins, diagnosis, reagents, elisa",

author = "P. Saldarelli and H.J.H.G. Keller and M. Dell'Orco and A. Schots and V. Elicio and A. Minafra",

year = "2005",

doi = "10.1016/j.jviromet.2004.11.021",

language = "English",

volume = "124",

pages = "191--195",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

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TY - JOUR

T1 - Isolation of recombinant antibodies (scFvs) to grapevine virus B

AU - Saldarelli, P.

AU - Keller, H.J.H.G.

AU - Dell'Orco, M.

AU - Schots, A.

AU - Elicio, V.

AU - Minafra, A.

PY - 2005

Y1 - 2005

N2 - A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.

AB - A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.

KW - phage display libraries

KW - fusion proteins

KW - diagnosis

KW - reagents

KW - elisa

U2 - 10.1016/j.jviromet.2004.11.021

DO - 10.1016/j.jviromet.2004.11.021

M3 - Article

SN - 0166-0934

VL - 124

SP - 191

EP - 195

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

Isolation of recombinant antibodies (scFvs) to grapevine virus B

Abstract

Keywords

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