Isolation of recombinant antibodies (scFvs) to grapevine virus B

P. Saldarelli, H.J.H.G. Keller, M. Dell'Orco, A. Schots, V. Elicio, A. Minafra

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.
Original languageEnglish
Pages (from-to)191-195
JournalJournal of Virological Methods
Volume124
Issue number1-2
DOIs
Publication statusPublished - 2005

Keywords

  • phage display libraries
  • fusion proteins
  • diagnosis
  • reagents
  • elisa

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