Isolation of recombinant antibodies (scFvs) to grapevine virus B

P. Saldarelli, H.J.H.G. Keller, M. Dell'Orco, A. Schots, V. Elicio, A. Minafra

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.
Original languageEnglish
Pages (from-to)191-195
JournalJournal of Virological Methods
Volume124
Issue number1-2
DOIs
Publication statusPublished - 2005

Fingerprint

Cercopithecine Herpesvirus 1
Single-Chain Antibodies
amsonic acid
Antibodies
Cell Surface Display Techniques
Virion
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Serum
Genes

Keywords

  • phage display libraries
  • fusion proteins
  • diagnosis
  • reagents
  • elisa

Cite this

Saldarelli, P., Keller, H. J. H. G., Dell'Orco, M., Schots, A., Elicio, V., & Minafra, A. (2005). Isolation of recombinant antibodies (scFvs) to grapevine virus B. Journal of Virological Methods, 124(1-2), 191-195. https://doi.org/10.1016/j.jviromet.2004.11.021
Saldarelli, P. ; Keller, H.J.H.G. ; Dell'Orco, M. ; Schots, A. ; Elicio, V. ; Minafra, A. / Isolation of recombinant antibodies (scFvs) to grapevine virus B. In: Journal of Virological Methods. 2005 ; Vol. 124, No. 1-2. pp. 191-195.
@article{175f903dfbf14b90983dfa5464be1850,
title = "Isolation of recombinant antibodies (scFvs) to grapevine virus B",
abstract = "A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.",
keywords = "phage display libraries, fusion proteins, diagnosis, reagents, elisa",
author = "P. Saldarelli and H.J.H.G. Keller and M. Dell'Orco and A. Schots and V. Elicio and A. Minafra",
year = "2005",
doi = "10.1016/j.jviromet.2004.11.021",
language = "English",
volume = "124",
pages = "191--195",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",
number = "1-2",

}

Saldarelli, P, Keller, HJHG, Dell'Orco, M, Schots, A, Elicio, V & Minafra, A 2005, 'Isolation of recombinant antibodies (scFvs) to grapevine virus B' Journal of Virological Methods, vol. 124, no. 1-2, pp. 191-195. https://doi.org/10.1016/j.jviromet.2004.11.021

Isolation of recombinant antibodies (scFvs) to grapevine virus B. / Saldarelli, P.; Keller, H.J.H.G.; Dell'Orco, M.; Schots, A.; Elicio, V.; Minafra, A.

In: Journal of Virological Methods, Vol. 124, No. 1-2, 2005, p. 191-195.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Isolation of recombinant antibodies (scFvs) to grapevine virus B

AU - Saldarelli, P.

AU - Keller, H.J.H.G.

AU - Dell'Orco, M.

AU - Schots, A.

AU - Elicio, V.

AU - Minafra, A.

PY - 2005

Y1 - 2005

N2 - A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.

AB - A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.

KW - phage display libraries

KW - fusion proteins

KW - diagnosis

KW - reagents

KW - elisa

U2 - 10.1016/j.jviromet.2004.11.021

DO - 10.1016/j.jviromet.2004.11.021

M3 - Article

VL - 124

SP - 191

EP - 195

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 1-2

ER -