Isolation of apoplastic fluid from leaf tissue by the vacuum infiltration-centrifugation technique

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

31 Citations (Scopus)


Upon infection of plants by pathogens, at least at the early stages of infection, the interaction between the two organisms occurs in the apoplast. To study the molecular basis of host susceptibility vs. resistance on the one hand, and pathogen virulence vs. avirulence on the other, the identification of extracellular compounds such as pathogen effectors that determine the outcome of the interaction is essential. Here, I describe the vacuum infiltration-centrifugation technique, which is an extremely simple and straightforward method to explore one of the most important battlefields of a plant–pathogen interaction; the apoplast.
Original languageEnglish
Title of host publicationPlant Fungal Pathogens: Methods and Protocols
EditorsM.D. Bolton, B.P.H.J. Thomma
Place of PublicationNew York, USA
Number of pages648
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, c/o Springer Science+Business Media


  • Apoplast
  • Apoplastic fluid
  • Avirulence
  • Communication
  • Effector
  • Extracellular space
  • Intercellular washing fluid
  • Plant-pathogen interaction
  • Resistance
  • Vacuum infiltration-centrifugation technique


Dive into the research topics of 'Isolation of apoplastic fluid from leaf tissue by the vacuum infiltration-centrifugation technique'. Together they form a unique fingerprint.

Cite this