Isolation, characterisation and expression patterns of a RAD51 ortholog from Pleurotus ostreatus

T.S.P. Mikosch, A.S.M. Sonnenberg, L.J.L.D. van Griensven

    Research output: Contribution to journalArticleAcademicpeer-review

    4 Citations (Scopus)

    Abstract

    AB: Using degenerated primers for conserved regions of RecA homologs we have isolated a gene from Pleurotus ostreatus that shows characteristic features of RAD51 homologs. The encoded amino acid sequence of P. ostreatus RAD51 (PoRAD51) shows greatest sequence similarities with RAD51 from Coprinus cinereus (89% identity). Furthermore the genomic organisation of PoRAD51 is almost identical to that of RAD51 from C. cinereus. Northern analysis shows that the expression of PoRAD51 is found in vegetative mycelium, and fruit body tissue, and that it is expressed at elevated levels in lamellae/basidia and following DNA damage. A sporulation deficient mutant strain of P. ostreatus (ATTC 58937) showed expression patterns of the RAD51 gene that are similar those of the normal sporulating strain.
    Original languageEnglish
    Pages (from-to)682-687
    JournalMycological Research
    Volume106
    Issue number6
    DOIs
    Publication statusPublished - 2002

    Keywords

    • saccharomyces-cerevisiae
    • genetic-recombination
    • coprinus-cinereus
    • strand exchange
    • reca homologs
    • protein
    • repair
    • dmc1
    • filament

    Fingerprint

    Dive into the research topics of 'Isolation, characterisation and expression patterns of a RAD51 ortholog from Pleurotus ostreatus'. Together they form a unique fingerprint.

    Cite this