Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.)

Manol Ognyanov; Connie Remoroza; Henk A. Schols; Yordan Georgiev; Maria Kratchanova; Christo Kratchanov

doi:10.1016/j.carbpol.2016.06.031

Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.)

Manol Ognyanov, Connie Remoroza, Henk A. Schols, Yordan Georgiev, Maria Kratchanova^*, Christo Kratchanov

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

39 Citations (Scopus)

Abstract

A pectic polysaccharide from rose hip (RH) fruits has been obtained by extraction with 1% aqueous citric acid. It was found that the polysaccharide fraction mainly consisted of galacturonic acid (45.5%) next to galactose (5.5%) and arabinose (4.7%). RH pectin is having a relatively high degree of methylesterification (62%) and acetylation (10%) and consists of different molecular weight populations in the range of 10-100 kDa. Enzymatic fingerprinting was performed using a combination of pectin lyase (PL) and endo-polygalacturonase. Detailed information about the structure and level of galacturonic acid oligomers released was obtained using LC-HILIC-MS/ELSD and HPAEC. Predominantly, unsaturated and methyl-esterified oligomers (DP 3-5) were released indicating that high proportions of methylesterified 'PL degradable' areas were present within the pectin. The data revealed that homogalacturonan is the main building block of the extracted pectin and consists of long methylesterified/acetylated GalA sequences interspersed with small blocks of non-methyl-esterified GalA units.

Original language	English
Pages (from-to)	803-811
Journal	Carbohydrate Polymers
Volume	151
DOIs	https://doi.org/10.1016/j.carbpol.2016.06.031
Publication status	Published - 2016

Keywords

endo-Polygalacturonase II
HILIC-MS/ELSD
Pectic polysaccharides
Pectin lyase
Rose hip

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10.1016/j.carbpol.2016.06.031

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@article{4a7736fef4eb4e7c9cb20387fc2e03f0,

title = "Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.)",

abstract = "A pectic polysaccharide from rose hip (RH) fruits has been obtained by extraction with 1% aqueous citric acid. It was found that the polysaccharide fraction mainly consisted of galacturonic acid (45.5%) next to galactose (5.5%) and arabinose (4.7%). RH pectin is having a relatively high degree of methylesterification (62%) and acetylation (10%) and consists of different molecular weight populations in the range of 10-100 kDa. Enzymatic fingerprinting was performed using a combination of pectin lyase (PL) and endo-polygalacturonase. Detailed information about the structure and level of galacturonic acid oligomers released was obtained using LC-HILIC-MS/ELSD and HPAEC. Predominantly, unsaturated and methyl-esterified oligomers (DP 3-5) were released indicating that high proportions of methylesterified 'PL degradable' areas were present within the pectin. The data revealed that homogalacturonan is the main building block of the extracted pectin and consists of long methylesterified/acetylated GalA sequences interspersed with small blocks of non-methyl-esterified GalA units.",

keywords = "endo-Polygalacturonase II, HILIC-MS/ELSD, Pectic polysaccharides, Pectin lyase, Rose hip",

author = "Manol Ognyanov and Connie Remoroza and Schols, {Henk A.} and Yordan Georgiev and Maria Kratchanova and Christo Kratchanov",

year = "2016",

doi = "10.1016/j.carbpol.2016.06.031",

language = "English",

volume = "151",

pages = "803--811",

journal = "Carbohydrate Polymers",

issn = "0144-8617",

publisher = "Elsevier",

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TY - JOUR

T1 - Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.)

AU - Ognyanov, Manol

AU - Remoroza, Connie

AU - Schols, Henk A.

AU - Georgiev, Yordan

AU - Kratchanova, Maria

AU - Kratchanov, Christo

PY - 2016

Y1 - 2016

N2 - A pectic polysaccharide from rose hip (RH) fruits has been obtained by extraction with 1% aqueous citric acid. It was found that the polysaccharide fraction mainly consisted of galacturonic acid (45.5%) next to galactose (5.5%) and arabinose (4.7%). RH pectin is having a relatively high degree of methylesterification (62%) and acetylation (10%) and consists of different molecular weight populations in the range of 10-100 kDa. Enzymatic fingerprinting was performed using a combination of pectin lyase (PL) and endo-polygalacturonase. Detailed information about the structure and level of galacturonic acid oligomers released was obtained using LC-HILIC-MS/ELSD and HPAEC. Predominantly, unsaturated and methyl-esterified oligomers (DP 3-5) were released indicating that high proportions of methylesterified 'PL degradable' areas were present within the pectin. The data revealed that homogalacturonan is the main building block of the extracted pectin and consists of long methylesterified/acetylated GalA sequences interspersed with small blocks of non-methyl-esterified GalA units.

AB - A pectic polysaccharide from rose hip (RH) fruits has been obtained by extraction with 1% aqueous citric acid. It was found that the polysaccharide fraction mainly consisted of galacturonic acid (45.5%) next to galactose (5.5%) and arabinose (4.7%). RH pectin is having a relatively high degree of methylesterification (62%) and acetylation (10%) and consists of different molecular weight populations in the range of 10-100 kDa. Enzymatic fingerprinting was performed using a combination of pectin lyase (PL) and endo-polygalacturonase. Detailed information about the structure and level of galacturonic acid oligomers released was obtained using LC-HILIC-MS/ELSD and HPAEC. Predominantly, unsaturated and methyl-esterified oligomers (DP 3-5) were released indicating that high proportions of methylesterified 'PL degradable' areas were present within the pectin. The data revealed that homogalacturonan is the main building block of the extracted pectin and consists of long methylesterified/acetylated GalA sequences interspersed with small blocks of non-methyl-esterified GalA units.

KW - endo-Polygalacturonase II

KW - HILIC-MS/ELSD

KW - Pectic polysaccharides

KW - Pectin lyase

KW - Rose hip

U2 - 10.1016/j.carbpol.2016.06.031

DO - 10.1016/j.carbpol.2016.06.031

M3 - Article

AN - SCOPUS:84975321898

SN - 0144-8617

VL - 151

SP - 803

EP - 811

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Isolation and structure elucidation of pectic polysaccharide from rose hip fruits (Rosa canina L.)

Abstract

Keywords

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