Isolation and identification of radical scavengers in olive tree (Olea europaea) wood

M. Pérez-Bonilla, S. Salido, T.A. van Beek, P.J. Linares-Palomino, J. Altarejos, M. Nogueras, A. Sánchez

Research output: Contribution to journalArticleAcademicpeer-review

88 Citations (Scopus)

Abstract

Several extracts of Olea europaea wood (Picual olive cultivar) were obtained with solvents of different polarity and their antioxidant activities determined. The active compounds were detected in fractions of an ethyl acetate extract using HPLC with on-line radical scavenging detection. After applying different separation techniques, hydroxytyrosol, tyrosol, cycloolivil, 7-deoxyloganic acid, oleuropein and ligustroside were isolated and characterized. Hydroxytyrosol showed a higher activity than the natural antioxidant rosmarinic acid in scavenging the DPPH model radical. Cycloolivil and oleuropein showed stronger activities than the synthetic antioxidant BHT against the same radical. Ligustroside, tyrosol and 7-deoxyloganic acid showed little activity. The latter compound has not been previously identified in the genus Olea.
Original languageEnglish
Pages (from-to)311-318
Number of pages8
JournalJournal of Chromatography. A, Including electrophoresis and other separation methods
Volume1112
Issue number1-2
DOIs
Publication statusPublished - 2006

Fingerprint

Olea
Wood
Antioxidants
Scavenging
Butylated Hydroxytoluene
High Pressure Liquid Chromatography
7-deoxyloganic acid
cycloolivil
4-hydroxyphenylethanol
3,4-dihydroxyphenylethanol
oleuropein

Keywords

  • phenolic-compounds
  • antioxidant activity
  • liquid-chromatography
  • ligustrum-lucidum
  • mass-spectrometry
  • online detection
  • oil
  • extracts
  • lignans
  • elucidation

Cite this

Pérez-Bonilla, M. ; Salido, S. ; van Beek, T.A. ; Linares-Palomino, P.J. ; Altarejos, J. ; Nogueras, M. ; Sánchez, A. / Isolation and identification of radical scavengers in olive tree (Olea europaea) wood. In: Journal of Chromatography. A, Including electrophoresis and other separation methods. 2006 ; Vol. 1112, No. 1-2. pp. 311-318.
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abstract = "Several extracts of Olea europaea wood (Picual olive cultivar) were obtained with solvents of different polarity and their antioxidant activities determined. The active compounds were detected in fractions of an ethyl acetate extract using HPLC with on-line radical scavenging detection. After applying different separation techniques, hydroxytyrosol, tyrosol, cycloolivil, 7-deoxyloganic acid, oleuropein and ligustroside were isolated and characterized. Hydroxytyrosol showed a higher activity than the natural antioxidant rosmarinic acid in scavenging the DPPH model radical. Cycloolivil and oleuropein showed stronger activities than the synthetic antioxidant BHT against the same radical. Ligustroside, tyrosol and 7-deoxyloganic acid showed little activity. The latter compound has not been previously identified in the genus Olea.",
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Isolation and identification of radical scavengers in olive tree (Olea europaea) wood. / Pérez-Bonilla, M.; Salido, S.; van Beek, T.A.; Linares-Palomino, P.J.; Altarejos, J.; Nogueras, M.; Sánchez, A.

In: Journal of Chromatography. A, Including electrophoresis and other separation methods, Vol. 1112, No. 1-2, 2006, p. 311-318.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Isolation and identification of radical scavengers in olive tree (Olea europaea) wood

AU - Pérez-Bonilla, M.

AU - Salido, S.

AU - van Beek, T.A.

AU - Linares-Palomino, P.J.

AU - Altarejos, J.

AU - Nogueras, M.

AU - Sánchez, A.

PY - 2006

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N2 - Several extracts of Olea europaea wood (Picual olive cultivar) were obtained with solvents of different polarity and their antioxidant activities determined. The active compounds were detected in fractions of an ethyl acetate extract using HPLC with on-line radical scavenging detection. After applying different separation techniques, hydroxytyrosol, tyrosol, cycloolivil, 7-deoxyloganic acid, oleuropein and ligustroside were isolated and characterized. Hydroxytyrosol showed a higher activity than the natural antioxidant rosmarinic acid in scavenging the DPPH model radical. Cycloolivil and oleuropein showed stronger activities than the synthetic antioxidant BHT against the same radical. Ligustroside, tyrosol and 7-deoxyloganic acid showed little activity. The latter compound has not been previously identified in the genus Olea.

AB - Several extracts of Olea europaea wood (Picual olive cultivar) were obtained with solvents of different polarity and their antioxidant activities determined. The active compounds were detected in fractions of an ethyl acetate extract using HPLC with on-line radical scavenging detection. After applying different separation techniques, hydroxytyrosol, tyrosol, cycloolivil, 7-deoxyloganic acid, oleuropein and ligustroside were isolated and characterized. Hydroxytyrosol showed a higher activity than the natural antioxidant rosmarinic acid in scavenging the DPPH model radical. Cycloolivil and oleuropein showed stronger activities than the synthetic antioxidant BHT against the same radical. Ligustroside, tyrosol and 7-deoxyloganic acid showed little activity. The latter compound has not been previously identified in the genus Olea.

KW - phenolic-compounds

KW - antioxidant activity

KW - liquid-chromatography

KW - ligustrum-lucidum

KW - mass-spectrometry

KW - online detection

KW - oil

KW - extracts

KW - lignans

KW - elucidation

U2 - 10.1016/j.chroma.2005.12.055

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M3 - Article

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EP - 318

JO - Journal of Chromatography. A, Including electrophoresis and other separation methods

JF - Journal of Chromatography. A, Including electrophoresis and other separation methods

SN - 0021-9673

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