Isolation and characterization of the omega-6 fatty acid desaturase (FAD2) gene family in the allohexaploid oil seed crop Crambe abyssinica Hochst

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Crambe (Crambe abyssinica Hochst ex. R. E. Fries) is an ideal crop for industrial oil production because of its high erucic acid content (C22:1, approx. 60 %) in its seed oil. The value of crambe oil can be improved by increasing C22:1 content or reducing polyunsaturated fatty acids (PUFA). The FAD2 gene plays a critical role in PUFA biosynthesis. To identify targets for breeding, we characterized FAD2 in crambe for copy number and expression profile. Seven copies of FAD2 were detected in allohexaploid crambe. Three genes (CaFAD2-C1, -C2 and -C3) were expressed in multiple tissues, including root, seedling, leaf, flower, bud and developing seeds. In developing seeds, the expression of these genes was upregulated with CaFAD2-C3, being expressed predominantly with a peak at 20 days after pollination. This gene is therefore a promising candidate gene for determining PUFA levels in seed oil. Four other FAD2 genes were considered to be “pseudo-genes” as they harbour internal stop codons and were not expressed. Among the six crambe varieties with consistent variation in oil composition, no nucleotide polymorphisms were found in the CaFAD2-C1, -C2 and -C3 genes. In seeds at 30 days after pollination, statistically significant expression level polymorphisms for only one gene, CaFAD2-C2, was found among the varieties. However, although significantly different, the difference in expression was small and did not explain the variation in oil composition. Given the absence of genetic variation in CaFAD2 genes in crambe breeding lines, breeding for high erucic acid content calls for a molecular breeding approach whereby mutations are chemically induced to increase the genetic variation.
Original languageEnglish
Pages (from-to)517-531
JournalMolecular Breeding
Volume32
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

Crambe Plant
Omega-6 Fatty Acids
Fatty Acid Desaturases
Crambe abyssinica
stearoyl-CoA desaturase
oilseed crops
omega-6 fatty acids
Seeds
Oils
Crambe (Brassicaceae)
Genes
genes
Unsaturated Fatty Acids
Breeding
polyunsaturated fatty acids
Pollination
erucic acid
seed oils
Crambe Sponge
oils

Keywords

  • brassica-napus l.
  • high oleic-acid
  • erucic-acid
  • expression
  • genome
  • biosynthesis
  • suppression
  • stability
  • evolution
  • varieties

Cite this

@article{45c0f82a7167495686c5868b213b8a27,
title = "Isolation and characterization of the omega-6 fatty acid desaturase (FAD2) gene family in the allohexaploid oil seed crop Crambe abyssinica Hochst",
abstract = "Crambe (Crambe abyssinica Hochst ex. R. E. Fries) is an ideal crop for industrial oil production because of its high erucic acid content (C22:1, approx. 60 {\%}) in its seed oil. The value of crambe oil can be improved by increasing C22:1 content or reducing polyunsaturated fatty acids (PUFA). The FAD2 gene plays a critical role in PUFA biosynthesis. To identify targets for breeding, we characterized FAD2 in crambe for copy number and expression profile. Seven copies of FAD2 were detected in allohexaploid crambe. Three genes (CaFAD2-C1, -C2 and -C3) were expressed in multiple tissues, including root, seedling, leaf, flower, bud and developing seeds. In developing seeds, the expression of these genes was upregulated with CaFAD2-C3, being expressed predominantly with a peak at 20 days after pollination. This gene is therefore a promising candidate gene for determining PUFA levels in seed oil. Four other FAD2 genes were considered to be “pseudo-genes” as they harbour internal stop codons and were not expressed. Among the six crambe varieties with consistent variation in oil composition, no nucleotide polymorphisms were found in the CaFAD2-C1, -C2 and -C3 genes. In seeds at 30 days after pollination, statistically significant expression level polymorphisms for only one gene, CaFAD2-C2, was found among the varieties. However, although significantly different, the difference in expression was small and did not explain the variation in oil composition. Given the absence of genetic variation in CaFAD2 genes in crambe breeding lines, breeding for high erucic acid content calls for a molecular breeding approach whereby mutations are chemically induced to increase the genetic variation.",
keywords = "brassica-napus l., high oleic-acid, erucic-acid, expression, genome, biosynthesis, suppression, stability, evolution, varieties",
author = "J. Cheng and E.M.J. Salentijn and {Huang Bangquan} and F.A. Krens and A.C. Dechesne and R.G.F. Visser and {van Loo}, E.N.",
year = "2013",
doi = "10.1007/s11032-013-9886-0",
language = "English",
volume = "32",
pages = "517--531",
journal = "Molecular Breeding",
issn = "1380-3743",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Isolation and characterization of the omega-6 fatty acid desaturase (FAD2) gene family in the allohexaploid oil seed crop Crambe abyssinica Hochst

AU - Cheng, J.

AU - Salentijn, E.M.J.

AU - Huang Bangquan, null

AU - Krens, F.A.

AU - Dechesne, A.C.

AU - Visser, R.G.F.

AU - van Loo, E.N.

PY - 2013

Y1 - 2013

N2 - Crambe (Crambe abyssinica Hochst ex. R. E. Fries) is an ideal crop for industrial oil production because of its high erucic acid content (C22:1, approx. 60 %) in its seed oil. The value of crambe oil can be improved by increasing C22:1 content or reducing polyunsaturated fatty acids (PUFA). The FAD2 gene plays a critical role in PUFA biosynthesis. To identify targets for breeding, we characterized FAD2 in crambe for copy number and expression profile. Seven copies of FAD2 were detected in allohexaploid crambe. Three genes (CaFAD2-C1, -C2 and -C3) were expressed in multiple tissues, including root, seedling, leaf, flower, bud and developing seeds. In developing seeds, the expression of these genes was upregulated with CaFAD2-C3, being expressed predominantly with a peak at 20 days after pollination. This gene is therefore a promising candidate gene for determining PUFA levels in seed oil. Four other FAD2 genes were considered to be “pseudo-genes” as they harbour internal stop codons and were not expressed. Among the six crambe varieties with consistent variation in oil composition, no nucleotide polymorphisms were found in the CaFAD2-C1, -C2 and -C3 genes. In seeds at 30 days after pollination, statistically significant expression level polymorphisms for only one gene, CaFAD2-C2, was found among the varieties. However, although significantly different, the difference in expression was small and did not explain the variation in oil composition. Given the absence of genetic variation in CaFAD2 genes in crambe breeding lines, breeding for high erucic acid content calls for a molecular breeding approach whereby mutations are chemically induced to increase the genetic variation.

AB - Crambe (Crambe abyssinica Hochst ex. R. E. Fries) is an ideal crop for industrial oil production because of its high erucic acid content (C22:1, approx. 60 %) in its seed oil. The value of crambe oil can be improved by increasing C22:1 content or reducing polyunsaturated fatty acids (PUFA). The FAD2 gene plays a critical role in PUFA biosynthesis. To identify targets for breeding, we characterized FAD2 in crambe for copy number and expression profile. Seven copies of FAD2 were detected in allohexaploid crambe. Three genes (CaFAD2-C1, -C2 and -C3) were expressed in multiple tissues, including root, seedling, leaf, flower, bud and developing seeds. In developing seeds, the expression of these genes was upregulated with CaFAD2-C3, being expressed predominantly with a peak at 20 days after pollination. This gene is therefore a promising candidate gene for determining PUFA levels in seed oil. Four other FAD2 genes were considered to be “pseudo-genes” as they harbour internal stop codons and were not expressed. Among the six crambe varieties with consistent variation in oil composition, no nucleotide polymorphisms were found in the CaFAD2-C1, -C2 and -C3 genes. In seeds at 30 days after pollination, statistically significant expression level polymorphisms for only one gene, CaFAD2-C2, was found among the varieties. However, although significantly different, the difference in expression was small and did not explain the variation in oil composition. Given the absence of genetic variation in CaFAD2 genes in crambe breeding lines, breeding for high erucic acid content calls for a molecular breeding approach whereby mutations are chemically induced to increase the genetic variation.

KW - brassica-napus l.

KW - high oleic-acid

KW - erucic-acid

KW - expression

KW - genome

KW - biosynthesis

KW - suppression

KW - stability

KW - evolution

KW - varieties

U2 - 10.1007/s11032-013-9886-0

DO - 10.1007/s11032-013-9886-0

M3 - Article

VL - 32

SP - 517

EP - 531

JO - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

IS - 3

ER -