Isolation and characterization of strong gene regulatory sequences from apple, Malus x domestica

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

For the strong expression of genes in plant tissue, the availability of specific gene regulatory sequences is desired. We cloned promoter and terminator sequences of an apple (Malus x domestica) ribulose biphosphate carboxylase small subunit gene (MdRbcS), which is known for its high expression and used gus reporter gene expression to test the regulatory activity of the isolated promoter and terminator sequences in transgenic tobacco. The MdRbcS promoter itself seemed to be less strong than the CaMV35S promoter when both used in combination with the nos terminator. However, the combination of the promoter and terminator of MdRbcS was able to drive gus to similar expression levels as the reference construct with CaMV35S promoter and nos terminator. This observation indicates the importance of the terminator sequence for gene expression. It is concluded that the combination of the MdRbcS promoter and terminator is a suitable regulatory sequence set for the expression of transgenes to a high level in plants and for intragenesis in apple specifically
Original languageEnglish
Pages (from-to)135-142
JournalTree Genetics and Genomes
Volume7
Issue number1
DOIs
Publication statusPublished - 2011

Fingerprint

Genetic Terminator Regions
regulatory sequences
Malus
Regulator Genes
apples
promoter regions
terminator regions
Gene Expression
gene expression
gene
Ribulose-Bisphosphate Carboxylase
Plant Genes
genes
tobacco
Transgenes
Reporter Genes
Tobacco
ribulose
Genes
reporter genes

Keywords

  • rbcs-3a gene
  • expression
  • plants
  • elements
  • agrobacterium
  • arabidopsis
  • cisgenesis
  • resistance
  • promoters
  • tissues

Cite this

@article{721be8c1c34c4391a8f3d61586d3e009,
title = "Isolation and characterization of strong gene regulatory sequences from apple, Malus x domestica",
abstract = "For the strong expression of genes in plant tissue, the availability of specific gene regulatory sequences is desired. We cloned promoter and terminator sequences of an apple (Malus x domestica) ribulose biphosphate carboxylase small subunit gene (MdRbcS), which is known for its high expression and used gus reporter gene expression to test the regulatory activity of the isolated promoter and terminator sequences in transgenic tobacco. The MdRbcS promoter itself seemed to be less strong than the CaMV35S promoter when both used in combination with the nos terminator. However, the combination of the promoter and terminator of MdRbcS was able to drive gus to similar expression levels as the reference construct with CaMV35S promoter and nos terminator. This observation indicates the importance of the terminator sequence for gene expression. It is concluded that the combination of the MdRbcS promoter and terminator is a suitable regulatory sequence set for the expression of transgenes to a high level in plants and for intragenesis in apple specifically",
keywords = "rbcs-3a gene, expression, plants, elements, agrobacterium, arabidopsis, cisgenesis, resistance, promoters, tissues",
author = "J.G. Schaart and I.E.M. Tinnenbroek and F.A. Krens",
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language = "English",
volume = "7",
pages = "135--142",
journal = "Tree Genetics and Genomes",
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Isolation and characterization of strong gene regulatory sequences from apple, Malus x domestica. / Schaart, J.G.; Tinnenbroek, I.E.M.; Krens, F.A.

In: Tree Genetics and Genomes, Vol. 7, No. 1, 2011, p. 135-142.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Isolation and characterization of strong gene regulatory sequences from apple, Malus x domestica

AU - Schaart, J.G.

AU - Tinnenbroek, I.E.M.

AU - Krens, F.A.

PY - 2011

Y1 - 2011

N2 - For the strong expression of genes in plant tissue, the availability of specific gene regulatory sequences is desired. We cloned promoter and terminator sequences of an apple (Malus x domestica) ribulose biphosphate carboxylase small subunit gene (MdRbcS), which is known for its high expression and used gus reporter gene expression to test the regulatory activity of the isolated promoter and terminator sequences in transgenic tobacco. The MdRbcS promoter itself seemed to be less strong than the CaMV35S promoter when both used in combination with the nos terminator. However, the combination of the promoter and terminator of MdRbcS was able to drive gus to similar expression levels as the reference construct with CaMV35S promoter and nos terminator. This observation indicates the importance of the terminator sequence for gene expression. It is concluded that the combination of the MdRbcS promoter and terminator is a suitable regulatory sequence set for the expression of transgenes to a high level in plants and for intragenesis in apple specifically

AB - For the strong expression of genes in plant tissue, the availability of specific gene regulatory sequences is desired. We cloned promoter and terminator sequences of an apple (Malus x domestica) ribulose biphosphate carboxylase small subunit gene (MdRbcS), which is known for its high expression and used gus reporter gene expression to test the regulatory activity of the isolated promoter and terminator sequences in transgenic tobacco. The MdRbcS promoter itself seemed to be less strong than the CaMV35S promoter when both used in combination with the nos terminator. However, the combination of the promoter and terminator of MdRbcS was able to drive gus to similar expression levels as the reference construct with CaMV35S promoter and nos terminator. This observation indicates the importance of the terminator sequence for gene expression. It is concluded that the combination of the MdRbcS promoter and terminator is a suitable regulatory sequence set for the expression of transgenes to a high level in plants and for intragenesis in apple specifically

KW - rbcs-3a gene

KW - expression

KW - plants

KW - elements

KW - agrobacterium

KW - arabidopsis

KW - cisgenesis

KW - resistance

KW - promoters

KW - tissues

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JO - Tree Genetics and Genomes

JF - Tree Genetics and Genomes

SN - 1614-2942

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