Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp.

A. Schwenzfeier, P.A. Wierenga, H. Gruppen

Research output: Contribution to journalArticleAcademicpeer-review

113 Citations (Scopus)

Abstract

Extraction of high-value protein fractions for techno-functional applications in foods can considerably increase the commercial value of microalgae biomass. Proteins from Tetraselmis sp. were extracted and purified after cell disintegration by bead milling, centrifugation, ion exchange chromatography using the absorbent Streamline DEAE, and final decolorization by precipitation at pH 3.5. The algae soluble isolate was free from the intense color typical for algae products and contained 64% (w/w) proteins and 24% (w/w) carbohydrates. The final isolate showed solubility independent of ionic strength and 100% solubility at and above pH 5.5. Since most plant proteins used in foods show poor solubility in the pH range 5.5–6.5, the algae soluble protein isolate could be useful for techno-functional applications in this pH range.
Original languageEnglish
Pages (from-to)9121-9127
JournalBioresource Technology
Volume102
Issue number19
DOIs
Publication statusPublished - 2011

Fingerprint

Algae
Proteins
protein
Solubility
solubility
alga
Plant Proteins
Centrifugation
Disintegration
Chromatography
Ionic strength
food
Ion exchange
Carbohydrates
Biomass
chromatography
carbohydrate
Color
ion exchange
biomass

Keywords

  • expanded-bed adsorption
  • marine microalgae
  • chromatography
  • chlorella
  • hydrolysis
  • glycinin
  • nitrogen
  • biomass
  • cells
  • acid

Cite this

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title = "Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp.",
abstract = "Extraction of high-value protein fractions for techno-functional applications in foods can considerably increase the commercial value of microalgae biomass. Proteins from Tetraselmis sp. were extracted and purified after cell disintegration by bead milling, centrifugation, ion exchange chromatography using the absorbent Streamline DEAE, and final decolorization by precipitation at pH 3.5. The algae soluble isolate was free from the intense color typical for algae products and contained 64{\%} (w/w) proteins and 24{\%} (w/w) carbohydrates. The final isolate showed solubility independent of ionic strength and 100{\%} solubility at and above pH 5.5. Since most plant proteins used in foods show poor solubility in the pH range 5.5–6.5, the algae soluble protein isolate could be useful for techno-functional applications in this pH range.",
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Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp. / Schwenzfeier, A.; Wierenga, P.A.; Gruppen, H.

In: Bioresource Technology, Vol. 102, No. 19, 2011, p. 9121-9127.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp.

AU - Schwenzfeier, A.

AU - Wierenga, P.A.

AU - Gruppen, H.

PY - 2011

Y1 - 2011

N2 - Extraction of high-value protein fractions for techno-functional applications in foods can considerably increase the commercial value of microalgae biomass. Proteins from Tetraselmis sp. were extracted and purified after cell disintegration by bead milling, centrifugation, ion exchange chromatography using the absorbent Streamline DEAE, and final decolorization by precipitation at pH 3.5. The algae soluble isolate was free from the intense color typical for algae products and contained 64% (w/w) proteins and 24% (w/w) carbohydrates. The final isolate showed solubility independent of ionic strength and 100% solubility at and above pH 5.5. Since most plant proteins used in foods show poor solubility in the pH range 5.5–6.5, the algae soluble protein isolate could be useful for techno-functional applications in this pH range.

AB - Extraction of high-value protein fractions for techno-functional applications in foods can considerably increase the commercial value of microalgae biomass. Proteins from Tetraselmis sp. were extracted and purified after cell disintegration by bead milling, centrifugation, ion exchange chromatography using the absorbent Streamline DEAE, and final decolorization by precipitation at pH 3.5. The algae soluble isolate was free from the intense color typical for algae products and contained 64% (w/w) proteins and 24% (w/w) carbohydrates. The final isolate showed solubility independent of ionic strength and 100% solubility at and above pH 5.5. Since most plant proteins used in foods show poor solubility in the pH range 5.5–6.5, the algae soluble protein isolate could be useful for techno-functional applications in this pH range.

KW - expanded-bed adsorption

KW - marine microalgae

KW - chromatography

KW - chlorella

KW - hydrolysis

KW - glycinin

KW - nitrogen

KW - biomass

KW - cells

KW - acid

U2 - 10.1016/j.biortech.2011.07.046

DO - 10.1016/j.biortech.2011.07.046

M3 - Article

VL - 102

SP - 9121

EP - 9127

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

IS - 19

ER -