Isolation and characterization of satellite cells from rat head branchiomeric muscles

P.L. Carvajal Monroy; Z. Yablonka-Reuveni; Sander Grefte; Anne Marie Kuijpers-Jagtman; F.A.D.T.G. Wagener; J.W. Von den Hoff

doi:10.3791/52802

Isolation and characterization of satellite cells from rat head branchiomeric muscles

P.L. Carvajal Monroy, Z. Yablonka-Reuveni, Sander Grefte, Anne Marie Kuijpers-Jagtman, F.A.D.T.G. Wagener, J.W. Von den Hoff^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.

Original language	English
Article number	e52802
Journal	Journal of Visualized Experiments
Volume	101
DOIs	https://doi.org/10.3791/52802
Publication status	Published - 20 Jul 2015

Keywords

satellite cells
rat head branchiomeric muscles

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10.3791/52802Licence: CC BY-NC-ND

https://edepot.wur.nl/371802Licence: CC BY-NC-ND

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title = "Isolation and characterization of satellite cells from rat head branchiomeric muscles",

abstract = "This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.",

keywords = "satellite cells, rat head branchiomeric muscles",

author = "{Carvajal Monroy}, P.L. and Z. Yablonka-Reuveni and Sander Grefte and Kuijpers-Jagtman, {Anne Marie} and F.A.D.T.G. Wagener and {Von den Hoff}, J.W.",

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AU - Carvajal Monroy, P.L.

AU - Yablonka-Reuveni, Z.

AU - Grefte, Sander

AU - Kuijpers-Jagtman, Anne Marie

AU - Wagener, F.A.D.T.G.

AU - Von den Hoff, J.W.

PY - 2015/7/20

Y1 - 2015/7/20

N2 - This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.

AB - This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.

KW - satellite cells

KW - rat head branchiomeric muscles

U2 - 10.3791/52802

DO - 10.3791/52802

M3 - Article

SN - 1940-087X

VL - 101

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

M1 - e52802

ER -

Isolation and characterization of satellite cells from rat head branchiomeric muscles

Abstract

Keywords

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