Isolation and characterization of microsatellite loci in Cylindrocladium pauciramosum

L.P. Wright, B.D. Wingfield, P.W. Crous, M.J. Wingfield

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Ten polymorphic microsatellite markers were developed for Cylindrocladium pauciramosum, a plant pathogen with a wide host range, which poses a serious problem in South African Eucalyptus nurseries. Polymorphism was evaluated on 43 isolates collected from Colombia and South Africa. Each locus had between three and six alleles. Testing for random mating showed multilocus equilibrium for a population of 40 isolates from a South African forestry nursery. Cross-species transferability tested for 19 other Cylindrocladium species found amplification only in C. spathulatum, which is phylogenetically closely related to C. pauciramosum.
Original languageEnglish
Pages (from-to)343-345
JournalMolecular Ecology Notes
Volume7
Issue number2
DOIs
Publication statusPublished - 2007

Fingerprint

Nurseries
Forestry
Pathogens
Polymorphism
Microsatellite Repeats
Amplification
Eucalyptus
Colombia
Host Specificity
host range
Testing
South Africa
amplification
allele
forestry
polymorphism
pathogen
Alleles
Population
marker

Keywords

  • anamorphs
  • vesicles

Cite this

Wright, L.P. ; Wingfield, B.D. ; Crous, P.W. ; Wingfield, M.J. / Isolation and characterization of microsatellite loci in Cylindrocladium pauciramosum. In: Molecular Ecology Notes. 2007 ; Vol. 7, No. 2. pp. 343-345.
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Isolation and characterization of microsatellite loci in Cylindrocladium pauciramosum. / Wright, L.P.; Wingfield, B.D.; Crous, P.W.; Wingfield, M.J.

In: Molecular Ecology Notes, Vol. 7, No. 2, 2007, p. 343-345.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Isolation and characterization of microsatellite loci in Cylindrocladium pauciramosum

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AU - Wingfield, B.D.

AU - Crous, P.W.

AU - Wingfield, M.J.

PY - 2007

Y1 - 2007

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AB - Ten polymorphic microsatellite markers were developed for Cylindrocladium pauciramosum, a plant pathogen with a wide host range, which poses a serious problem in South African Eucalyptus nurseries. Polymorphism was evaluated on 43 isolates collected from Colombia and South Africa. Each locus had between three and six alleles. Testing for random mating showed multilocus equilibrium for a population of 40 isolates from a South African forestry nursery. Cross-species transferability tested for 19 other Cylindrocladium species found amplification only in C. spathulatum, which is phylogenetically closely related to C. pauciramosum.

KW - anamorphs

KW - vesicles

U2 - 10.1111/j.1471-8286.2006.01603.x

DO - 10.1111/j.1471-8286.2006.01603.x

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JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

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