Isolation and characterization of a novel potato Auxin/Indole-3Acetic Acid family member (StAA2) that is involved in petiole hyponasty and shoot morphogenesis

B.A. Kloosterman, R.G.F. Visser, C.W.B. Bachem

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25 Citations (Scopus)

Abstract

Auxin/indole-3-acetid acid (Aux/IAA) proteins are short-lived transcriptional regulators that mediate their response through interaction with auxin response factors (ARF). Although 29 Aux/IAA proteins have been identified in Arabidopsis thaliana, their individual functions are still poorly understood and are largely defined by observed growth defects in gain-of-function mutant alleles. Here we present the isolation and characterization of a novel Aux/IAA protein in potato (Solanum tuberosum) that is named StIAA2. Down regulation of StIAA2 results in distinctive phenotypes that include, increased plant height, petiole hyponasty and extreme curvature of growing leaf primordia in the shoot apex. Gene expression analysis of transgenic plants with reduced StIAA2 transcript levels resulted in the identification of a number of genes with altered expression profiles including another member of the Aux/IAA gene family (StIAA). The phenotypes that were observed in the StIAA2 suppression clones can be associated with both common as well as unique functional roles among Aux/IAA family members indicating the importance of analyzing Aux/IAA expression in different plant species
Original languageEnglish
Pages (from-to)766-775
JournalPlant Physiology and Biochemistry
Volume44
Issue number11-12
DOIs
Publication statusPublished - 2006

Fingerprint

Indoleacetic Acids
indoles
Solanum tuberosum
Morphogenesis
morphogenesis
auxins
potatoes
Acids
shoots
acids
potato protein
Phenotype
phenotype
leaf primordia
Proteins
Genetically Modified Plants
indole
Arabidopsis
Genes
transgenic plants

Keywords

  • brassinosteroid-regulated genes
  • of-function mutation
  • aux/iaa proteins
  • auxin-response
  • tuber development
  • root development
  • plant hormones
  • abscisic-acid
  • expression
  • arabidopsis

Cite this

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title = "Isolation and characterization of a novel potato Auxin/Indole-3Acetic Acid family member (StAA2) that is involved in petiole hyponasty and shoot morphogenesis",
abstract = "Auxin/indole-3-acetid acid (Aux/IAA) proteins are short-lived transcriptional regulators that mediate their response through interaction with auxin response factors (ARF). Although 29 Aux/IAA proteins have been identified in Arabidopsis thaliana, their individual functions are still poorly understood and are largely defined by observed growth defects in gain-of-function mutant alleles. Here we present the isolation and characterization of a novel Aux/IAA protein in potato (Solanum tuberosum) that is named StIAA2. Down regulation of StIAA2 results in distinctive phenotypes that include, increased plant height, petiole hyponasty and extreme curvature of growing leaf primordia in the shoot apex. Gene expression analysis of transgenic plants with reduced StIAA2 transcript levels resulted in the identification of a number of genes with altered expression profiles including another member of the Aux/IAA gene family (StIAA). The phenotypes that were observed in the StIAA2 suppression clones can be associated with both common as well as unique functional roles among Aux/IAA family members indicating the importance of analyzing Aux/IAA expression in different plant species",
keywords = "brassinosteroid-regulated genes, of-function mutation, aux/iaa proteins, auxin-response, tuber development, root development, plant hormones, abscisic-acid, expression, arabidopsis",
author = "B.A. Kloosterman and R.G.F. Visser and C.W.B. Bachem",
year = "2006",
doi = "10.1016/j.plaphy.2006.10.026",
language = "English",
volume = "44",
pages = "766--775",
journal = "Plant Physiology and Biochemistry",
issn = "0981-9428",
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TY - JOUR

T1 - Isolation and characterization of a novel potato Auxin/Indole-3Acetic Acid family member (StAA2) that is involved in petiole hyponasty and shoot morphogenesis

AU - Kloosterman, B.A.

AU - Visser, R.G.F.

AU - Bachem, C.W.B.

PY - 2006

Y1 - 2006

N2 - Auxin/indole-3-acetid acid (Aux/IAA) proteins are short-lived transcriptional regulators that mediate their response through interaction with auxin response factors (ARF). Although 29 Aux/IAA proteins have been identified in Arabidopsis thaliana, their individual functions are still poorly understood and are largely defined by observed growth defects in gain-of-function mutant alleles. Here we present the isolation and characterization of a novel Aux/IAA protein in potato (Solanum tuberosum) that is named StIAA2. Down regulation of StIAA2 results in distinctive phenotypes that include, increased plant height, petiole hyponasty and extreme curvature of growing leaf primordia in the shoot apex. Gene expression analysis of transgenic plants with reduced StIAA2 transcript levels resulted in the identification of a number of genes with altered expression profiles including another member of the Aux/IAA gene family (StIAA). The phenotypes that were observed in the StIAA2 suppression clones can be associated with both common as well as unique functional roles among Aux/IAA family members indicating the importance of analyzing Aux/IAA expression in different plant species

AB - Auxin/indole-3-acetid acid (Aux/IAA) proteins are short-lived transcriptional regulators that mediate their response through interaction with auxin response factors (ARF). Although 29 Aux/IAA proteins have been identified in Arabidopsis thaliana, their individual functions are still poorly understood and are largely defined by observed growth defects in gain-of-function mutant alleles. Here we present the isolation and characterization of a novel Aux/IAA protein in potato (Solanum tuberosum) that is named StIAA2. Down regulation of StIAA2 results in distinctive phenotypes that include, increased plant height, petiole hyponasty and extreme curvature of growing leaf primordia in the shoot apex. Gene expression analysis of transgenic plants with reduced StIAA2 transcript levels resulted in the identification of a number of genes with altered expression profiles including another member of the Aux/IAA gene family (StIAA). The phenotypes that were observed in the StIAA2 suppression clones can be associated with both common as well as unique functional roles among Aux/IAA family members indicating the importance of analyzing Aux/IAA expression in different plant species

KW - brassinosteroid-regulated genes

KW - of-function mutation

KW - aux/iaa proteins

KW - auxin-response

KW - tuber development

KW - root development

KW - plant hormones

KW - abscisic-acid

KW - expression

KW - arabidopsis

U2 - 10.1016/j.plaphy.2006.10.026

DO - 10.1016/j.plaphy.2006.10.026

M3 - Article

VL - 44

SP - 766

EP - 775

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

SN - 0981-9428

IS - 11-12

ER -