Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey

M. Balk, G.H.J. Heilig, M.H.A. van Eekert, A.J.M. Stams, W.I.C. Rijpstra, J.S. Sinninghe-Damsté, W.M. de Vos, S.W.M. Kengen

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17 Citations (Scopus)

Abstract

A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Aya¿, Turkey. The cells were straight to curved rods, 0.4-0.6 mum in diameter and 3.5-10 mum in length. Spores were terminal and round. The temperature range for growth was 40-80 degrees C, with an optimum at 70 degrees C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H(2), and CO(2). Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H(2) and CO(2) formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA-DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans
Original languageEnglish
Pages (from-to)885-894
JournalExtremophiles
Volume13
Issue number6
DOIs
Publication statusPublished - 2009

Keywords

  • anaerobic thermophilic bacterium
  • yellowstone-national-park
  • sp-nov.
  • gen.-nov.
  • thermoterrabacterium-ferrireducens
  • carboxydothermus-hydrogenoformans
  • clostridium-thermosulfurogenes
  • hydrogenogenic bacterium
  • renaturation rates
  • dna hybridization

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