Isolation and characterisation of a strawberry fruit-specific promotor

J.G. Schaart, E.M.J. Salentijn, K.T.B. Pelgrom, A. Aharoni, F.A. Krens

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In order to achieve specific expression of transgenes in strawberry fruits, the availability of tissue- (receptacle) specific promoter sequences is desired. For this reason, 5'-upstream sequences of the strawberry expansin gene FaExp2, which is expressed in a fruit-specific manner, have been isolated. To characterise the promoter activity of the isolated sequences, fragments of 0.7 kb (0.7pFaExp2)and 1.6 kb (1.6pFaExp2) have been fused to the ß-glucuronidase reporter gene (gus). In transgenic strawberry plants transformed with either 0.7pFaExp2-gus or1.6pFaExp2-gus, a fruit-specific expression pattern was observed for both promoter constructs. However, quantitative RT-PCR revealed that gus expression levels driven by the 1.6pFaExp2 promoter fragment were much more higher. In addition to the expression in fruits, both promoter fragments also seemed to direct gene expression in the achenes and to some extent in epidermal and subepidermal tissues of petioles and stems of flowers and fruits. It is concluded that both promoter sequences are suitable for directing transgene expression in strawberry fruits in a specific way
Original languageEnglish
Pages (from-to)108-114
JournalGenes, Genomes and Genomics
Volume5
Issue numberSpecial Issue 1
Publication statusPublished - 2011

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strawberries
promoter regions
fruits
transgenes
expansins
petioles
reporter genes
reverse transcriptase polymerase chain reaction
genetically modified organisms
flowers
gene expression
stems
genes

Cite this

@article{c3b03118d2f04e4c8b7fb8199386243d,
title = "Isolation and characterisation of a strawberry fruit-specific promotor",
abstract = "In order to achieve specific expression of transgenes in strawberry fruits, the availability of tissue- (receptacle) specific promoter sequences is desired. For this reason, 5'-upstream sequences of the strawberry expansin gene FaExp2, which is expressed in a fruit-specific manner, have been isolated. To characterise the promoter activity of the isolated sequences, fragments of 0.7 kb (0.7pFaExp2)and 1.6 kb (1.6pFaExp2) have been fused to the {\ss}-glucuronidase reporter gene (gus). In transgenic strawberry plants transformed with either 0.7pFaExp2-gus or1.6pFaExp2-gus, a fruit-specific expression pattern was observed for both promoter constructs. However, quantitative RT-PCR revealed that gus expression levels driven by the 1.6pFaExp2 promoter fragment were much more higher. In addition to the expression in fruits, both promoter fragments also seemed to direct gene expression in the achenes and to some extent in epidermal and subepidermal tissues of petioles and stems of flowers and fruits. It is concluded that both promoter sequences are suitable for directing transgene expression in strawberry fruits in a specific way",
author = "J.G. Schaart and E.M.J. Salentijn and K.T.B. Pelgrom and A. Aharoni and F.A. Krens",
year = "2011",
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pages = "108--114",
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}

Isolation and characterisation of a strawberry fruit-specific promotor. / Schaart, J.G.; Salentijn, E.M.J.; Pelgrom, K.T.B.; Aharoni, A.; Krens, F.A.

In: Genes, Genomes and Genomics, Vol. 5, No. Special Issue 1, 2011, p. 108-114.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Schaart, J.G.

AU - Salentijn, E.M.J.

AU - Pelgrom, K.T.B.

AU - Aharoni, A.

AU - Krens, F.A.

PY - 2011

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AB - In order to achieve specific expression of transgenes in strawberry fruits, the availability of tissue- (receptacle) specific promoter sequences is desired. For this reason, 5'-upstream sequences of the strawberry expansin gene FaExp2, which is expressed in a fruit-specific manner, have been isolated. To characterise the promoter activity of the isolated sequences, fragments of 0.7 kb (0.7pFaExp2)and 1.6 kb (1.6pFaExp2) have been fused to the ß-glucuronidase reporter gene (gus). In transgenic strawberry plants transformed with either 0.7pFaExp2-gus or1.6pFaExp2-gus, a fruit-specific expression pattern was observed for both promoter constructs. However, quantitative RT-PCR revealed that gus expression levels driven by the 1.6pFaExp2 promoter fragment were much more higher. In addition to the expression in fruits, both promoter fragments also seemed to direct gene expression in the achenes and to some extent in epidermal and subepidermal tissues of petioles and stems of flowers and fruits. It is concluded that both promoter sequences are suitable for directing transgene expression in strawberry fruits in a specific way

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