Involvement of phenazines and lipopeptides in interactions between Pseudomonas species and Sclerotium rolfsii, causal agent of stem rot disease on groundnut

N.C. Le, M. Kruijt, J. Raaijmakers

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

Aims: To determine the role of phenazines (PHZ) and lipopeptide surfactants (LPs) produced by Pseudomonas in suppression of stem rot disease of groundnut, caused by the fungal pathogen Sclerotium rolfsii. Methods and Results: In vitro assays showed that PHZ-producing Pseudomonas chlororaphis strain Phz24 significantly inhibited hyphal growth of S. rolfsii and suppressed stem rot disease of groundnut under field conditions. Biosynthesis and regulatory mutants of Phz24 deficient in PHZ production were less effective in pathogen suppression. Pseudomonas strains SS101, SBW25, and 267, producing viscosin or putisolvin-like LPs, only marginally inhibited hyphal growth of S. rolfsii and did not suppress stem rot disease. In contrast, Pseudomonas strain SH-C52, producing the chlorinated LP thanamycin, inhibited hyphal growth of S. rolfsii and significantly reduced stem rot disease of groundnut in nethouse and field experiments, whereas its thanamycin-deficient mutant was less effective. Conclusions: Phenazines and specific lipopeptides play an important role in suppression of stem rot disease of groundnut by root-colonizing Pseudomonas strains. Significance and Impact of the Study: Pseudomonas strains Phz24 and SH-C52 showed significant control of stem rot disease. Treatment of seeds or soil with these strains provides a promising supplementary strategy to control stem rot disease of groundnut
Original languageEnglish
Pages (from-to)390-403
JournalJournal of Applied Microbiology
Volume112
Issue number2
DOIs
Publication statusPublished - 2012

Keywords

  • rhizoctonia root-rot
  • arachis-hypogaea l.
  • biological-control
  • fluorescent pseudomonads
  • antibiotic production
  • biocontrol agents
  • biofilm formation
  • in-vitro
  • biosynthesis
  • peanut

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