Involvement of ethylene and lipid signalling in cadmium-induced programmed cell death in tomato suspension cells

E.T. Iakimova, V.M. Kapchina-Toteva, L.J. Laarhoven, F. Harren, E.J. Woltering

    Research output: Contribution to journalArticleAcademicpeer-review

    73 Citations (Scopus)

    Abstract

    Cadmium-induced cell death was studied in suspension-cultured tomato (Lycopersicon esculentum Mill.) cells (line MsK8) treated with CdSO4. Within 24 h, cadmium treatment induced cell death in a concentration-dependent manner. Cell cultures showed recovery after 2¿3 days which indicates the existence of an adaptation mechanism. Cadmium-induced cell death was alleviated by the addition of sub ¿M concentrations of peptide inhibitors specific to human caspases indicating that cell death proceeds through a mechanism with similarities to animal programmed cell death (PCD, apoptosis). Cadmium-induced cell death was accompanied by an increased production of hydrogen peroxide (H2O2) and simultaneous addition of antioxidants greatly reduced cell death. Inhibitors of phospholipase C (PLC) and phospholipase D (PLD) signalling pathway intermediates reduced cadmium-induced cell death. Treatment with the G-protein activator mastoparan and a cell permeable analogue of the lipid signal second messenger phosphatidic acid (PA) induced cell death. Ethylene, while not inducing cell death when applied alone, stimulated cadmium-induced cell death. Application of the ethylene biosynthesis inhibitor aminoethoxy vinylglycine (AVG) reduced cadmium-induced cell death, and this effect was alleviated by simultaneous treatment with ethylene. Together the results show that cadmium induces PCD exhibiting apoptotic-like features. The cell death process requires increased H2O2 production and activation of PLC, PLD and ethylene signalling pathways
    Original languageEnglish
    Pages (from-to)581-589
    JournalPlant Physiology and Biochemistry
    Volume44
    Issue number10
    DOIs
    Publication statusPublished - 2006

    Keywords

    • caspase-like activity
    • phosphatidic-acid
    • hydrogen-peroxide
    • tobacco cells
    • induced apoptosis
    • plants
    • activation
    • release
    • stress
    • phytochelatins

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