Investigation of Hepatitis A and e viruses in mussels collected from the bosphorus, in Istanbul, Turkey - Short communication

Huseyin Yilmaz*, Utku Cizmecigil, Eda Altan Tarakci, Ozge Aydin, Aysun Yilmaz, Mehmet Calicioglu, Gurhan Ciftcioglu, Ali Aydin, Kamil Bostan, Tansel Sireli, Mine Guzel, Asiye Karakullukcu, Bekir Kocazeybek, Wim Van Der Poel, Juergen Richt, Nuri Turan

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Hepatitis A and E viruses (HAV and HEV) are recognised as food- and water-borne infections and shellfish are a well-documented source of these viruses. The presence of HAV and HEV in mussels has not previously been investigated in Bosphorus, Istanbul, Turkey, and therefore the aim of this study was to investigate the frequency of HAV and HEV in mussels collected from this location. A total of 92 pooled samples representing 736 mussels originating along the Bosphorus coast were collected from fish distributors. RNA was extracted using the RNeasy Plant Mini Kit and a TaqMan real-time RT-PCR was performed using primers specific to HAV and HEV. Amongst the 92 pooled samples tested, three (3.3%) were found to be positive for HAV by the TaqMan real-time RT-PCR. No HEV was detected in any of the mussels tested. A 174 bp product was observed on gel electrophoresis with HAV positive samples detected by SYBR Green real-time PCR after reverse transcription. This product was sequenced and confirmed to contain HAV sequences by alignment using the data in GenBank. This is the first report describing the presence of HAV in mussels harvested in the Bosphorus, Istanbul, Turkey, indicating the potential contamination of aquatic environment and risk to public health.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalCzech Journal of Food Sciences
Volume36
Issue number3
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Hepatitis
  • Mussels
  • Real-time RT-PCR
  • Turkey
  • Virus

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